• Western blot analysis of extracts of HCT116 cells, using CD133 antibody (STJ114584) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit . Exposure time: 30s.
  • Western blot analysis of extracts from normal (control) and CD133 Rabbit pAb knockout (KO) HCT116 cells, using CD133 Rabbit pAb antibody (STJ114584) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit . Exposure time: 30s.
  • Immunofluorescence analysis of NIH/3T3 cells using CD133 antibody (STJ114584) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of U-2 OS cells using CD133 antibody (STJ114584) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Immunoprecipitation analysis of 200ug extracts of HT-29 cells using 3ug CD133 antibody (STJ114584). Western blot was performed from the immunoprecipitate using CD133 antibody (STJ114584) at a dilition of 1:1000.

Anti-CD133-antibody (aa750-850) (STJ114584)

SKU:
STJ114584

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Host: Rabbit
Applications: WB, IF, IP
Reactivity: Human, Mouse, Rat
Note: FOR RESEARCH USE ONLY (RUO).
Short Description: Rabbit polyclonal antibody anti-CD133 is suitable for use in Western Blot, Immunofluorescence and Immunoprecipitation.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:1000-1:3000
IF 1:50-1:200
IP 1:50-1:200
Storage Instruction: Store at-20°C. Avoid freeze/thaw cycles.
Gene Symbol: PROM1
Gene ID: 8842
Uniprot ID: PROM1_HUMAN
Immunogen Region: 750-850
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 750-850 of human CD133 (NP_006008.1).
Tissue Specificity Isoform 1 is selectively expressed on CD34 hematopoietic stem and progenitor cells in adult and fetal bone marrow, fetal liver, cord blood and adult peripheral blood. Isoform 1 is not detected on other blood cells. Isoform 1 is also expressed in a number of non-lymphoid tissues including retina, pancreas, placenta, kidney, liver, lung, brain and heart. Found in saliva within small membrane particles. Isoform 2 is predominantly expressed in fetal liver, skeletal muscle, kidney, and heart as well as adult pancreas, kidney, liver, lung, and placenta. Isoform 2 is highly expressed in fetal liver, low in bone marrow, and barely detectable in peripheral blood. Isoform 2 is expressed on hematopoietic stem cells and in epidermal basal cells (at protein level). Expressed in adult retina by rod and cone photoreceptor cells (at protein level).
Post Translational Modifications Isoform 1 and isoform 2 are glycosylated. Acetylation at Lys-225, Lys-257 and Lys-264 by NAT8 and NAT8B may control PROM1 protein expression and its function in cell apoptosis.
Function May play a role in cell differentiation, proliferation and apoptosis. Binds cholesterol in cholesterol-containing plasma membrane microdomains and may play a role in the organization of the apical plasma membrane in epithelial cells. During early retinal development acts as a key regulator of disk morphogenesis. Involved in regulation of MAPK and Akt signaling pathways. In neuroblastoma cells suppresses cell differentiation such as neurite outgrowth in a RET-dependent manner.
Protein Name Prominin-1
Antigen Ac133
Prominin-Like Protein 1
Cd Antigen Cd133
Cellular Localisation Apical Cell Membrane
Multi-Pass Membrane Protein
Cell Projection
Microvillus Membrane
Cilium
Photoreceptor Outer Segment
Endoplasmic Reticulum
Endoplasmic Reticulum-Golgi Intermediate Compartment
Found In Extracellular Membrane Particles In Various Body Fluids Such As Cerebrospinal Fluid
Saliva
Seminal Fluid And Urine
Alternative Antibody Names Anti-Prominin-1 antibody
Anti-Antigen Ac133 antibody
Anti-Prominin-Like Protein 1 antibody
Anti-Cd Antigen Cd133 antibody
Anti-PROM1 antibody
Anti-PROML1 antibody
Anti-MSTP061 antibody

Information sourced from Uniprot.org

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