• Immunoprecipitation analysis of 200 Mu g extracts of HepG2 cells using 1 Mu g CBX4 antibody (STJ27483). Western blot was performed from the immunoprecipitate using CBX4 antibody (STJ27483) at a dilution of 1:500.
  • Western blot analysis of various lysates using CBX4 Rabbit polyclonal antibody (STJ27483) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-CBX4 antibody (401-560) (STJ27483)

SKU:
STJ27483

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IP
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-CBX4 (401-560) is suitable for use in Western Blot and Immunoprecipitation research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IP 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CBX4
Gene ID: 8535
Uniprot ID: CBX4_HUMAN
Immunogen Region: 401-560
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 401-560 of human CBX4 (NP_003646.2).
Immunogen Sequence: AVGLNLSHVRKRCLSETHGE REPCKKRLTARSISTPTCLG GSPAAERPADLPPAAALPQP EVILLDSDLDEPIDLRCVKT RSEAGEPPSSLQVKPETPAS AAVAVAAAAAPTTTAEKPPA EAQDEPAESLSEFKPFFGNI IITDVTANCLTVTFKEYVTV
Tissue Specificity Ubiquitous.
Post Translational Modifications Ubiquitinated. Ubiquitination regulates the function of the Polycomb group (PcG) multiprotein PRC1-like complex. Deubiquitinated by USP26. Phosphorylated on Thr-497 by HIPK2 upon DNA damage. This phosphorylation stimulates E3 SUMO-protein ligase activity and promotes sumoylation on Lys-494, as well as sumoylation of other target proteins, such as HNRNPK.
Function E3 SUMO-protein ligase which facilitates SUMO1 conjugation by UBE2I. Involved in the sumoylation of HNRNPK, a p53/TP53 transcriptional coactivator, hence indirectly regulates p53/TP53 transcriptional activation resulting in p21/CDKN1A expression. Monosumoylates ZNF131. Component of a Polycomb group (PcG) multiprotein PRC1-like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones.it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility. Binds to histone H3 trimethylated at 'Lys-9' (H3K9me3). Plays a role in the lineage differentiation of the germ layers in embryonic development.
Protein Name E3 Sumo-Protein Ligase Cbx4
Chromobox Protein Homolog 4
Polycomb 2 Homolog
Pc2
Hpc2
Database Links Reactome: R-HSA-2559580
Reactome: R-HSA-3108214
Reactome: R-HSA-3899300
Reactome: R-HSA-4551638
Reactome: R-HSA-4570464
Reactome: R-HSA-4655427
Reactome: R-HSA-8939243
Reactome: R-HSA-8943724
Cellular Localisation Nucleus
Nucleus Speckle
Localization To Nuclear Polycomb Bodies Is Required For Znf131 Sumoylation
Localized In Distinct Foci On Chromatin
Alternative Antibody Names Anti-E3 Sumo-Protein Ligase Cbx4 antibody
Anti-Chromobox Protein Homolog 4 antibody
Anti-Polycomb 2 Homolog antibody
Anti-Pc2 antibody
Anti-Hpc2 antibody
Anti-CBX4 antibody

Information sourced from Uniprot.org

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