• Western blot analysis of extracts of mouse testis, using CALY antibody (STJ112523) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-CALY antibody (1-87) (STJ112523)

SKU:
STJ112523

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Host: Rabbit
Applications: WB
Reactivity: Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-CALY (1-87) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CALY
Gene ID: 50632
Uniprot ID: CALY_HUMAN
Immunogen Region: 1-87
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-87 of human CALY (NP_056537.1).
Immunogen Sequence: MVKLGCSFSGKPGKDPGDQD GAAMDSVPLISPLDISQLQP PLPDQVVIKTQTEYQLSSPD QQNFPDLEGQRLNCSHPEEG RRLPTAR
Tissue Specificity Expressed in the pyramidal cells of the prefrontal cortex, in hypothalamus and in caudate nucleus. No expression in spleen. Up-regulated in the prefrontal cortex of schizophrenic patients with nearly twice the levels of non-schizophrenics.
Post Translational Modifications Glycosylated.
Function Interacts with clathrin light chain A and stimulates clathrin self-assembly and clathrin-mediated endocytosis.
Protein Name Neuron-Specific Vesicular Protein Calcyon
Cellular Localisation Cytoplasmic Vesicle Membrane
Single-Pass Membrane Protein
Cell Membrane
Alternative Antibody Names Anti-Neuron-Specific Vesicular Protein Calcyon antibody
Anti-CALY antibody
Anti-DRD1IP antibody

Information sourced from Uniprot.org

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