Immunohistochemical analysis of paraffin-embedded Human stomach. 1, Antibody was diluted at 1:100 (4°C overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 30min).

Anti-CA9 antibody [12F10] (STJ96978)

SKU:
STJ96978
£48.00 - £252.00
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Host: Mouse
Applications: IF/WB/IHC/IP
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description : Mouse monoclonal antibody anti-Carbonic anhydrase 9 is suitable for use in Immunofluorescence, Western Blot, Immunohistochemistry and Immunoprecipitation research applications.
Clonality : Monoclonal
Clone ID : 12F10
Conjugation: Unconjugated
Isotype: IgG1
Formulation: Liquid in PBS pH7.4, 0.5% BSA, 0.02% Sodium Azide and 50% Glycerol.
Purification: The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
Dilution Range: IF 1:50-200
WB 1:3000
IP 1:200
IHC 1:50-300
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CA9
Gene ID: 768
Uniprot ID: CAH9_HUMAN
Specificity: The antibody detects endogenous CA IX proteins.
Immunogen: Synthetic Peptide of CA IX
Post Translational Modifications Asn-346 bears high-mannose type glycan structures.
Function Catalyzes the interconversion between carbon dioxide and water and the dissociated ions of carbonic acid (i.e. bicarbonate and hydrogen ions).
Protein Name Carbonic Anhydrase 9
Carbonate Dehydratase Ix
Carbonic Anhydrase Ix
Ca-Ix
Caix
Membrane Antigen Mn
P54/58n
Renal Cell Carcinoma-Associated Antigen G250
Rcc-Associated Antigen G250
Pmw1
Database Links Reactome: R-HSA-1234158
Reactome: R-HSA-1475029
Cellular Localisation Nucleus
Nucleolus
Cell Membrane
Single-Pass Type I Membrane Protein
Cell Projection
Microvillus Membrane
Found On The Surface Microvilli And In The Nucleus
Particularly In Nucleolus
Alternative Antibody Names Anti-Carbonic Anhydrase 9 antibody
Anti-Carbonate Dehydratase Ix antibody
Anti-Carbonic Anhydrase Ix antibody
Anti-Ca-Ix antibody
Anti-Caix antibody
Anti-Membrane Antigen Mn antibody
Anti-P54/58n antibody
Anti-Renal Cell Carcinoma-Associated Antigen G250 antibody
Anti-Rcc-Associated Antigen G250 antibody
Anti-Pmw1 antibody
Anti-CA9 antibody
Anti-G250 antibody
Anti-MN antibody

Information sourced from Uniprot.org

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