• Immunofluorescence analysis of U-2 OS cells using BZW2 Rabbit polyclonal antibody (STJ118268) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of L929 cells using BZW2 Rabbit polyclonal antibody (STJ118268) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Western blot analysis of various lysates using BZW2 Rabbit polyclonal antibody (STJ118268) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-BZW2 antibody (190-300) (STJ118268)

SKU:
STJ118268

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-BZW2 (190-300) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: BZW2
Gene ID: 28969
Uniprot ID: 5MP1_HUMAN
Immunogen Region: 190-300
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 190-300 of human BZW2 (NP_054757.1).
Immunogen Sequence: LFKAWMAEKDANSVTSSLRK ANLDKRLLELFPVNRQSVDH FAKYFTDAGLKELSDFLRVQ QSLGTRKELQKELQERLSQE CPIKEVVLYVKEEMKRNDLP ETAVIGLLWTC
Function Translation initiation regulator which represses non-AUG initiated translation and repeat-associated non-AUG (RAN) initiated translation by acting as a competitive inhibitor of eukaryotic translation initiation factor 5 (EIF5) function. Increases the accuracy of translation initiation by impeding EIF5-dependent translation from non-AUG codons by competing with it for interaction with EIF2S2 within the 43S pre-initiation complex (PIC) in an EIF3C-binding dependent manner.
Protein Name Eif5-Mimic Protein 1
Basic Leucine Zipper And W2 Domain-Containing Protein 2
Cellular Localisation Cytoplasm
Alternative Antibody Names Anti-Eif5-Mimic Protein 1 antibody
Anti-Basic Leucine Zipper And W2 Domain-Containing Protein 2 antibody
Anti-BZW2 antibody
Anti-5MP1 antibody
Anti-HSPC028 antibody
Anti-MSTP017 antibody

Information sourced from Uniprot.org

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