• Western blot analysis of extracts of various cell lines, using BUD31 antibody (STJ114097) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 30s.

Anti-BUD31 antibody (1-144) (STJ114097)

SKU:
STJ114097

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-BUD31 (1-144) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: BUD31
Gene ID: 8896
Uniprot ID: BUD31_HUMAN
Immunogen Region: 1-144
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-144 of human BUD31 (NP_003901.2).
Immunogen Sequence: MPKVKRSRKAPPDGWELIEP TLDELDQKMREAETEPHEGK RKVESLWPIFRIHHQKTRYI FDLFYKRKAISRELYEYCIK EGYADKNLIAKWKKQGYENL CCLRCIQTRDTNFGTNCICR VPKSKLEVGRIIECTHCGCR GCSG
Tissue Specificity Detected in epithelial and stromal cells in benign prostate hyperplasia tissue (at protein level).
Function Involved in the pre-mRNA splicing process. May play a role as regulator of AR transcriptional activity.may increase AR transcriptional activity.
Protein Name Protein Bud31 Homolog
Protein Edg-2
Protein G10 Homolog
Database Links Reactome: R-HSA-72163
Cellular Localisation Nucleus
Detected In Chromatin At The Promoter Of Ar Target Genes
Alternative Antibody Names Anti-Protein Bud31 Homolog antibody
Anti-Protein Edg-2 antibody
Anti-Protein G10 Homolog antibody
Anti-BUD31 antibody
Anti-EDG2 antibody

Information sourced from Uniprot.org

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