• Immunofluorescence analysis of mouse lung using BTN3A1 Polyclonal Antibody (STJ112326) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of rat lung using BTN3A1 Polyclonal Antibody (STJ112326) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Western blot analysis of extracts of various cell lines, using BTN3A1 antibody (STJ112326) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Anti-BTN3A1 antibody (40-250) (STJ112326)

SKU:
STJ112326

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-BTN3A1 (40-250) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:100
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: BTN3A1
Gene ID: 11119
Uniprot ID: BT3A1_HUMAN
Immunogen Region: 40-250
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 40-250 of human BTN3A1 (NP_008979.3).
Immunogen Sequence: ILAMVGEDADLPCHLFPTMS AETMELKWVSSSLRQVVNVY ADGKEVEDRQSAPYRGRTSI LRDGITAGKAALRIHNVTAS DSGKYLCYFQDGDFYEKALV ELKVAALGSDLHVDVKGYKD GGIHLECRSTGWYPQPQIQW SNNKGENIPTVEAPVVADGV GLYAVAASVIMRGSSGEGVS CTIRSSLLGLEKTASISIAD PFFRSAQRWIA
Tissue Specificity Detected on T-cells, natural killer cells, dendritic cells and macrophages (at protein level). Ubiquitous. Highly expressed in heart, pancreas and lung, Moderately expressed in placenta, liver and muscle.
Post Translational Modifications N-glycosylated.
Function Plays a role in T-cell activation and in the adaptive immune response. Regulates the proliferation of activated T-cells. Regulates the release of cytokines and IFNG by activated T-cells. Mediates the response of T-cells toward infected and transformed cells that are characterized by high levels of phosphorylated metabolites, such as isopentenyl pyrophosphate.
Protein Name Butyrophilin Subfamily 3 Member A1
Cd Antigen Cd277
Database Links Reactome: R-HSA-8851680
Cellular Localisation Cell Membrane
Single-Pass Type I Membrane Protein
Alternative Antibody Names Anti-Butyrophilin Subfamily 3 Member A1 antibody
Anti-Cd Antigen Cd277 antibody
Anti-BTN3A1 antibody
Anti-BTF5 antibody

Information sourced from Uniprot.org

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