• Chromatin immunoprecipitation analysis of extracts of HeLa cells, using BTAF1 antibody (STJ28374) and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.
  • Immunoprecipitation analysis of 150 Mu g extracts of HeLa cells using 3 Mu g BTAF1 antibody (STJ28374). Western blot was performed from the immunoprecipitate using BTAF1 antibody (STJ28374) at a dilution of 1:500.
  • Western blot analysis of various lysates using BTAF1 Rabbit polyclonal antibody (STJ28374) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit.

Anti-BTAF1 antibody (1600-1849) (STJ28374)

SKU:
STJ28374

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IP
Reactivity: Human/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-BTAF1 (1600-1849) is suitable for use in Western Blot and Immunoprecipitation research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IP 1:20-1:50
ChIP 1:20-1:100
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: BTAF1
Gene ID: 9044
Uniprot ID: BTAF1_HUMAN
Immunogen Region: 1600-1849
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1600-1849 of human BTAF1 (NP_003963.1).
Immunogen Sequence: LAVQNSSLHDIQHAPKLSAL KQLLLDCGLGNGSTSESGTE SVVAQHRILIFCQLKSMLDI VEHDLLKPHLPSVTYLRLDG SIPPGQRHSIVSRFNNDPSI DVLLLTTHVGGLGLNLTGAD TVVFVEHDWNPMRDLQAMDR AHRIGQKRVVNVYRLITRGT LEEKIMGLQKFKMNIANTVI SQENSSLQSMGTDQLLDLFT LDKDGKAEKADTSTSGKASM KSILENLSDLWDQEQYDSE
Function Regulates transcription in association with TATA binding protein (TBP). Removes TBP from the TATA box in an ATP-dependent manner.
Protein Name Tata-Binding Protein-Associated Factor 172
Atp-Dependent Helicase Btaf1
B-Tfiid Transcription Factor-Associated 170 Kda Subunit
Taf(Ii170
Tbp-Associated Factor 172
Taf-172
Cellular Localisation Nucleus
Alternative Antibody Names Anti-Tata-Binding Protein-Associated Factor 172 antibody
Anti-Atp-Dependent Helicase Btaf1 antibody
Anti-B-Tfiid Transcription Factor-Associated 170 Kda Subunit antibody
Anti-Taf(Ii170 antibody
Anti-Tbp-Associated Factor 172 antibody
Anti-Taf-172 antibody
Anti-BTAF1 antibody
Anti-TAF172 antibody

Information sourced from Uniprot.org

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