| Host: | Rabbit |
| Applications: | WB/IF/ICC/ELISA |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Purification: | Affinity purification |
| Concentration: | Lot specific |
| Dilution Range: | WB:1:100-1:500 IF/ICC:1:50-1:200 ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | BRCA1 |
| Gene ID: | 672 |
| Uniprot ID: | BRCA1_HUMAN |
| Immunogen Region: | 400-1863 |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 400-699 of human BRCA1 (NP_009225.1). |
| Immunogen Sequence: | DGESESNAKVADVLDVLNEV DEYSGSSEKIDLLASDPHEA LICKSERVHSKSVESNIEDK IFGKTYRKKASLPNLSHVTE NLIIGAFVTEPQIIQERPLT NKLKRKRRPTSGLHPEDFIK KADLAVQKTPEMINQGTNQT EQNGQVMNITNSGHENKTKG DSIQNEKNPNPIESLEKESA FKTKAEPISSSISNMELELN IHNSKAPKKNRLRRKSSTRH IHALELVVSRNLSPPNCTE |
| Tissue Specificity | Isoform 1 and isoform 3 are widely expressed. Isoform 3 is reduced or absent in several breast and ovarian cancer cell lines. |
| Post Translational Modifications | Phosphorylated in response to IR, UV, and various stimuli that cause checkpoint activation, probably by ATM or ATR. Phosphorylation at Ser-988 by CHEK2 regulates mitotic spindle assembly. Phosphorylation by AURKA regulates centrosomal microtubule nucleation. Autoubiquitinated, undergoes 'Lys-6'-linked polyubiquitination. 'Lys-6'-linked polyubiquitination does not promote degradation. |
| Function | E3 ubiquitin-protein ligase that specifically mediates the formation of 'Lys-6'-linked polyubiquitin chains and plays a central role in DNA repair by facilitating cellular responses to DNA damage. It is unclear whether it also mediates the formation of other types of polyubiquitin chains. The BRCA1-BARD1 heterodimer coordinates a diverse range of cellular pathways such as DNA damage repair, ubiquitination and transcriptional regulation to maintain genomic stability. Regulates centrosomal microtubule nucleation. Required for appropriate cell cycle arrests after ionizing irradiation in both the S-phase and the G2 phase of the cell cycle. Required for FANCD2 targeting to sites of DNA damage. Inhibits lipid synthesis by binding to inactive phosphorylated ACACA and preventing its dephosphorylation. Contributes to homologous recombination repair (HRR) via its direct interaction with PALB2, fine-tunes recombinational repair partly through its modulatory role in the PALB2-dependent loading of BRCA2-RAD51 repair machinery at DNA breaks. Component of the BRCA1-RBBP8 complex which regulates CHEK1 activation and controls cell cycle G2/M checkpoints on DNA damage via BRCA1-mediated ubiquitination of RBBP8. Acts as a transcriptional activator. |
| Protein Name | Breast Cancer Type 1 Susceptibility Protein Ring Finger Protein 53 Ring-Type E3 Ubiquitin Transferase Brca1 |
| Database Links | Reactome: R-HSA-1221632 Reactome: R-HSA-3108214 Reactome: R-HSA-5685938 Reactome: R-HSA-5685942 Reactome: R-HSA-5689901 Reactome: R-HSA-5693554 Reactome: R-HSA-5693565 Reactome: R-HSA-5693568 Reactome: R-HSA-5693571 Reactome: R-HSA-5693579 Reactome: R-HSA-5693607 Reactome: R-HSA-5693616 Reactome: R-HSA-6796648 Reactome: R-HSA-6804756 Reactome: R-HSA-69473 Reactome: R-HSA-8951664 Reactome: R-HSA-8953750 Reactome: R-HSA-912446 Reactome: R-HSA-9663199 Reactome: R-HSA-9699150 Reactome: R-HSA-9701192 Reactome: R-HSA-9704331 Reactome: R-HSA-9704646 Reactome: R-HSA-9709570 Reactome: R-HSA-9709603 Reactome: R-HSA-9755511 |
| Cellular Localisation | Nucleus Chromosome Cytoplasm Localizes At Sites Of Dna Damage At Double-Strand Breaks (Dsbs) Recruitment To Dna Damage Sites Is Mediated By Abraxas1 And The Brca1-A Complex Translocated To The Cytoplasm During Uv-Induced Apoptosis Isoform 3: Cytoplasm Isoform 5: Cytoplasm |
| Alternative Antibody Names | Anti-Breast Cancer Type 1 Susceptibility Protein antibody Anti-Ring Finger Protein 53 antibody Anti-Ring-Type E3 Ubiquitin Transferase Brca1 antibody Anti-BRCA1 antibody Anti-RNF53 antibody |
Information sourced from Uniprot.org