| Host: |
Rabbit |
| Applications: |
WB/IF |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-BLMH (1-300) is suitable for use in Western Blot and Immunofluorescence research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:2000IF/ICC 1:20-1:50 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
BLMH |
| Gene ID: |
642 |
| Uniprot ID: |
BLMH_HUMAN |
| Immunogen Region: |
1-300 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human BLMH (NP_000377.1). |
| Immunogen Sequence: |
MSSSGLNSEKVAALIQKLNS DPQFVLAQNVGTTHDLLDIC LKRATVQRAQHVFQHAVPQE GKPITNQKSSGRCWIFSCLN VMRLPFMKKLNIEEFEFSQS YLFFWDKVERCYFFLSAFVD TAQRKEPEDGRLVQFLLMNP ANDGGQWDMLVNIVEKYGVI PKKCFPESYTTEATRRMNDI LNHKMREFCIRLRNLVHSGA TKGEISATQDVMMEEIFRVV CICLGNPPETFTWEYRDKD |
| Function | The normal physiological role of BLM hydrolase is unknown, but it catalyzes the inactivation of the antitumor drug BLM (a glycopeptide) by hydrolyzing the carboxamide bond of its B-aminoalaninamide moiety thus protecting normal and malignant cells from BLM toxicity. |
| Protein Name | Bleomycin HydrolaseBhBlm HydrolaseBmh |
| Database Links | Reactome: R-HSA-983168 |
| Cellular Localisation | CytoplasmCytoplasmic GranuleCo-Localizes With Nudt12 In The Cytoplasmic Granules |
| Alternative Antibody Names | Anti-Bleomycin Hydrolase antibodyAnti-Bh antibodyAnti-Blm Hydrolase antibodyAnti-Bmh antibodyAnti-BLMH antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
![Western blot analysis of lysates from wild type (WT) and BUB1 knockout (KO) HeLa cells, using [KO Validated] BUB1 Rabbit polyclonal antibody (STJ11100026) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1min.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/300x300/products/89098/357557/STJ11100026_1__69013.1713121681.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and BUB1 knockout (KO) HeLa cells, using [KO Validated] BUB1 Rabbit polyclonal antibody (STJ11100026) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1min.")
