• Immunofluorescence analysis of THP-1 cells using Bcl-2 Rabbit polyclonal antibody (STJ113759) at a dilution of 1:150 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of Bcl-2 in paraffin-embedded human tonsil tissue using Bcl-2 Rabbit polyclonal antibody (STJ113759) at a dilution of 1:200 (40x lens). Microwave antigen retrieval was performed with 0. 01 M PBS buffer (pH 7. 2±0. 2) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of Bcl-2 in paraffin-embedded rat brain tissue using Bcl-2 Rabbit polyclonal antibody (STJ113759) at a dilution of 1:200 (40x lens). Microwave antigen retrieval was performed with 0. 01 M PBS buffer (pH 7. 2±0. 2) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of Bcl-2 in paraffin-embedded mouse brain tissue using Bcl-2 Rabbit polyclonal antibody (STJ113759) at a dilution of 1:200 (40x lens). Microwave antigen retrieval was performed with 0. 01 M PBS buffer (pH 7. 2±0. 2) prior to immunohistochemistry staining.
  • Western blot analysis of various lysates using Bcl-2 Rabbit polyclonal antibody (STJ113759) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-BCL2 antibody (1-239) (STJ113759)

SKU:
STJ113759

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Bcl-2 (1-239) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: BCL2
Gene ID: 596
Uniprot ID: BCL2_HUMAN
Immunogen Region: 1-239
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-239 of human Bcl-2 (NP_000624.2).
Immunogen Sequence: MAHAGRTGYDNREIVMKYIH YKLSQRGYEWDAGDVGAAPP GAAPAPGIFSSQPGHTPHPA ASRDPVARTSPLQTPAAPGA AAGPALSPVPPVVHLTLRQA GDDFSRRYRRDFAEMSSQLH LTPFTARGRFATVVEELFRD GVNWGRIVAFFEFGGVMCVE SVNREMSPLVDNIALWMTEY LNRHLHTWIQDNGGWDAFVE LYGPSMRPLFDFSWLSLKTL LSLALVGACITLGAYLGHK
Tissue Specificity Expressed in a variety of tissues.
Post Translational Modifications Phosphorylation/dephosphorylation on Ser-70 regulates anti-apoptotic activity. Growth factor-stimulated phosphorylation on Ser-70 by PKC is required for the anti-apoptosis activity and occurs during the G2/M phase of the cell cycle. In the absence of growth factors, BCL2 appears to be phosphorylated by other protein kinases such as ERKs and stress-activated kinases. Phosphorylated by MAPK8/JNK1 at Thr-69, Ser-70 and Ser-87, wich stimulates starvation-induced autophag. Dephosphorylated by protein phosphatase 2A (PP2A). Proteolytically cleaved by caspases during apoptosis. The cleaved protein, lacking the BH4 motif, has pro-apoptotic activity, causes the release of cytochrome c into the cytosol promoting further caspase activity. Monoubiquitinated by PRKN, leading to an increase in its stability. Ubiquitinated by SCF(FBXO10), leading to its degradation by the proteasome. Ubiquitinated by XIAP, leading to its degradation by the proteasome.
Function Suppresses apoptosis in a variety of cell systems including factor-dependent lymphohematopoietic and neural cells. Regulates cell death by controlling the mitochondrial membrane permeability. Appears to function in a feedback loop system with caspases. Inhibits caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor (APAF-1). Also acts as an inhibitor of autophagy: interacts with BECN1 and AMBRA1 during non-starvation conditions and inhibits their autophagy function. May attenuate inflammation by impairing NLRP1-inflammasome activation, hence CASP1 activation and IL1B release.
Protein Name Apoptosis Regulator Bcl-2
Database Links Reactome: R-HSA-111447
Reactome: R-HSA-111453
Reactome: R-HSA-6785807
Reactome: R-HSA-844455
Reactome: R-HSA-9018519
Reactome: R-HSA-9634638
Cellular Localisation Mitochondrion Outer Membrane
Single-Pass Membrane Protein
Nucleus Membrane
Endoplasmic Reticulum Membrane
Cytoplasm
Alternative Antibody Names Anti-Apoptosis Regulator Bcl-2 antibody
Anti-BCL2 antibody

Information sourced from Uniprot.org

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