• Immunohistochemical analysis of paraffin-embedded Human breast cancer. 1, Using Bax Mouse mAb ( (STJ97729) diluted at 1:200 (4°C overnight). 2, High-pressure and temperature Citric acid, pH6.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 50min). Picture was kindly provided by our customer from Tianjin Medical University Cancer Institute and Hospital
  • Western blot detection of Bax in human breast cancer cell line MCF-7 (A), MDA-MB-231 (B) and Cal51 (C) using Bax mouse mAb ( (STJ97729, 1:1000 diluted).Predicted band size: 20kDa.Observed band size:20kDa. Picture was kindly provided by our customer from Tianjin Medical University Cancer Institute and Hospital
  • Immunohistochemical analysis of paraffin-embedded Mouse Kidney Tissue using Bax Mouse mAb diluted at 1:200.
  • Immunohistochemical analysis of paraffin-embedded Human Breast Carcinoma Tissue using Bax Mouse mAb diluted at 1:200.
  • Immunohistochemical analysis of paraffin-embedded Rat Testis Tissue using Bax Mouse mAb diluted at 1:200.

Anti-BAX antibody (30-110 aa) [1C1] (STJ97729)

SKU:
STJ97729

£46.00 - £226.00
Current Stock:
Host: Mouse
Applications: WB/IHC/IF
Reactivity: Human/Rat/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Mouse monoclonal antibody anti-Apoptosis regulator BAX (30-110 aa) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Monoclonal
Clone ID: 1C1
Conjugation: Unconjugated
Isotype: IgG1
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:1000-2000
IHC 1:100-200
IF 1:50-200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: BAX
Gene ID: 581
Uniprot ID: BAX_HUMAN
Immunogen Region: 30-110 aa
Specificity: Bax protein detects endogenous levels of BAX
Immunogen: Synthetic peptide of Bax at the amino acid range of 30-110
Post Translational Modifications Ubiquitinated on Lys-128 and Lys-190. 'Lys-63'-linked polyubiquitin chains on Lys-128 are removed by USP12.
Function Plays a role in the mitochondrial apoptotic process. Under normal conditions, BAX is largely cytosolic via constant retrotranslocation from mitochondria to the cytosol mediated by BCL2L1/Bcl-xL, which avoids accumulation of toxic BAX levels at the mitochondrial outer membrane (MOM). Under stress conditions, undergoes a conformation change that causes translocation to the mitochondrion membrane, leading to the release of cytochrome c that then triggers apoptosis. Promotes activation of CASP3, and thereby apoptosis.
Protein Name Apoptosis Regulator Bax
Bcl-2-Like Protein 4
Bcl2-L-4
Database Links Reactome: R-HSA-111457
Reactome: R-HSA-114294
Reactome: R-HSA-5620971
Reactome: R-HSA-6803204
Reactome: R-HSA-6804114
Reactome: R-HSA-8878166
Reactome: R-HSA-9603505
Cellular Localisation Isoform Alpha: Mitochondrion Outer Membrane
Single-Pass Membrane Protein
Cytoplasm
Nucleus
Colocalizes With 14-3-3 Proteins In The Cytoplasm
Under Stress Conditions
Undergoes A Conformation Change That Causes Release From Jnk-Phosphorylated 14-3-3 Proteins And Translocation To The Mitochondrion Membrane
Upon Sendai Virus Infection
Recruited To The Mitochondrion Through Interaction With Irf3
Isoform Beta: Cytoplasm
Isoform Gamma: Cytoplasm
Isoform Delta: Cytoplasm
Alternative Antibody Names Anti-Apoptosis Regulator Bax antibody
Anti-Bcl-2-Like Protein 4 antibody
Anti-Bcl2-L-4 antibody
Anti-BAX antibody
Anti-BCL2L4 antibody

Information sourced from Uniprot.org

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