• Western blot analysis of extracts of A-431 cells, using BAGE2 antibody (STJ112198) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-BAGE2 antibody (18-109) (STJ112198)

SKU:
STJ112198

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Host: Rabbit
Applications: WB
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-BAGE2 (18-109) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: BAGE2Uniprot ID=BAGE2_HUMAN"
Immunogen Region: 18-109
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 18-109 of human BAGE2 (NP_872288.2).
Immunogen Sequence: RLMKEESPVVSWRLEPEDGT ALDVHFVSTLEPLSNAVKRN VPRCIIILVLQEPTPFRISV TSSCFVQNTLTKLLKDRRKM QTVQCATARETS
Tissue Specificity Not expressed in normal tissues except in testis. Expressed in 22% of melanomas, in bladder and lung carcinomas.
Function Unknown. Candidate gene encoding tumor antigens.
Protein Name B Melanoma Antigen 2
Cancer/Testis Antigen 2.2
Ct2.2
Cellular Localisation Secreted
Alternative Antibody Names Anti-B Melanoma Antigen 2 antibody
Anti-Cancer/Testis Antigen 2.2 antibody
Anti-Ct2.2 antibody
Anti-BAGE2 antibody

Information sourced from Uniprot.org

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