• Immunofluorescence analysis of HeLa cells using Bad antibody (STJ22755). Blue: DAPI for nuclear staining.
  • Western blot analysis of extracts of various cell lines, using Bad antibody (STJ22755) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-BAD antibody (50-150) (STJ22755)

SKU:
STJ22755

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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-BAD (50-150) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:10-1:100
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: BAD
Gene ID: 572
Uniprot ID: BAD_HUMAN
Immunogen Region: 50-150
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 50-150 of human Bad (NP_004313.1).
Immunogen Sequence: HQQEQPTSSSHHGGAGAVEI RSRHSSYPAGTEDDEGMGEE PSPFRGRSRSAPPNLWAAQR YGRELRRMSDEFVDSFKKGL PRPKSAGTATQMRQSSSWTR V
Tissue Specificity Expressed in a wide variety of tissues.
Post Translational Modifications Phosphorylated on one or more of Ser-75, Ser-99, Ser-118 and Ser-134 in response to survival stimuli, which blocks its pro-apoptotic activity. Phosphorylation on Ser-99 or Ser-75 promotes heterodimerization with 14-3-3 proteins. This interaction then facilitates the phosphorylation at Ser-118, a site within the BH3 motif, leading to the release of Bcl-X(L) and the promotion of cell survival. Ser-99 is the major site of AKT/PKB phosphorylation, Ser-118 the major site of protein kinase A (CAPK) phosphorylation. Phosphorylation at Ser-99 by PKB/AKT1 is almost completely blocked by the apoptotic C-terminus cleavage product of PKN2 generated by caspases-3 activity during apoptosis. Methylation at Arg-94 and Arg-96 by PRMT1 inhibits Akt-mediated phosphorylation at Ser-99.
Function Promotes cell death. Successfully competes for the binding to Bcl-X(L), Bcl-2 and Bcl-W, thereby affecting the level of heterodimerization of these proteins with BAX. Can reverse the death repressor activity of Bcl-X(L), but not that of Bcl-2. Appears to act as a link between growth factor receptor signaling and the apoptotic pathways.
Protein Name Bcl2-Associated Agonist Of Cell Death
Bad
Bcl-2-Binding Component 6
Bcl-2-Like Protein 8
Bcl2-L-8
Bcl-Xl/Bcl-2-Associated Death Promoter
Bcl2 Antagonist Of Cell Death
Database Links Reactome: R-HSA-111447
Reactome: R-HSA-111453
Reactome: R-HSA-193648
Reactome: R-HSA-198323
Reactome: R-HSA-5674400
Cellular Localisation Mitochondrion Outer Membrane
Cytoplasm
Colocalizes With Hif3a In The Cytoplasm
Upon Phosphorylation
Locates To The Cytoplasm
Alternative Antibody Names Anti-Bcl2-Associated Agonist Of Cell Death antibody
Anti-Bad antibody
Anti-Bcl-2-Binding Component 6 antibody
Anti-Bcl-2-Like Protein 8 antibody
Anti-Bcl2-L-8 antibody
Anti-Bcl-Xl/Bcl-2-Associated Death Promoter antibody
Anti-Bcl2 Antagonist Of Cell Death antibody
Anti-BAD antibody
Anti-BBC6 antibody
Anti-BCL2L8 antibody

Information sourced from Uniprot.org

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