• Western blot analysis of extracts of various cell lines, using B4GALNT1 antibody (STJ116220) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Anti-B4GALNT1 antibody (26-240) (STJ116220)

SKU:
STJ116220

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Host: Rabbit
Applications: WB
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-B4GALNT1 (26-240) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: B4GALNT1
Gene ID: 2583
Uniprot ID: B4GN1_HUMAN
Immunogen Region: 26-240
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 26-240 of human B4GALNT1 (NP_001263398.1).
Immunogen Sequence: STRDAPGLRLPLAPWAPPQS PRRPELPDLAPEPRYAHIPV RIKEQVVGLLAWNNCSCESS GGGLPLPFQKQVRAIDLTKA FDPAELRAASATREQEFQAF LSRSQSPADQLLIAPANSPL QYPLQGVEVQPLRSILVPGL SLQAASGQEVYQVNLTASLG TWDVAGEVTGVTLTGEGQAD LTLVSPGLDQLNRQLQLVTY SSRSYQTNTADTGAR
Function Involved in the biosynthesis of gangliosides GM2, GD2, GT2 and GA2 from GM3, GD3, GT3 and GA3, respectively.
Protein Name Beta-1 -4 N-Acetylgalactosaminyltransferase 1
(N-Acetylneuraminyl-Galactosylglucosylceramide
Gm2/Gd2 Synthase
Galnac-T
Database Links Reactome: R-HSA-1660662
Cellular Localisation Golgi Apparatus Membrane
Single-Pass Type Ii Membrane Protein
Alternative Antibody Names Anti-Beta-1 -4 N-Acetylgalactosaminyltransferase 1 antibody
Anti-(N-Acetylneuraminyl-Galactosylglucosylceramide antibody
Anti-Gm2/Gd2 Synthase antibody
Anti-Galnac-T antibody
Anti-B4GALNT1 antibody
Anti-GALGT antibody
Anti-SIAT2 antibody

Information sourced from Uniprot.org

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