• Immunofluorescence analysis of L929 cells using B3GNT4 Rabbit polyclonal antibody (STJ118341) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of HeLa cells using B3GNT4 Rabbit polyclonal antibody (STJ118341) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of C6 cells using B3GNT4 Rabbit polyclonal antibody (STJ118341) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Western blot analysis of various lysates using B3GNT4 Rabbit polyclonal antibody (STJ118341) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Anti-B3GNT4 antibody (165-305) (STJ118341)

SKU:
STJ118341

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-B3GNT4 (165-305) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: B3GNT4
Gene ID: 79369
Uniprot ID: B3GN4_HUMAN
Immunogen Region: 165-305
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 165-305 of human B3GNT4 (NP_110392.1).
Immunogen Sequence: APPAQLLAYESREFDDILQW DFTEDFFNLTLKELHLQRWV VAACPQAHFMLKGDDDVFVH VPNVLEFLDGWDPAQDLLVG DVIRQALPNRNTKVKYFIPP SMYRATHYPPYAGGGGYVMS RATVRRLQAIMEDAELFPID D
Tissue Specificity Mainly expressed in brain tissues such as whole brain, hippocampus, amygdala, cerebellum and caudate nucleus. Also expressed in colon, esophagus and kidney.
Function Beta-1,3-N-acetylglucosaminyltransferase involved in the synthesis of poly-N-acetyllactosamine. Has activity for type 2 oligosaccharides.
Protein Name N-Acetyllactosaminide Beta-1 -3-N-Acetylglucosaminyltransferase 4
Udp-Glcnac -Betagal Beta-1 -3-N-Acetylglucosaminyltransferase 4
Bgnt-4
Beta-1 -3-Gn-T4
Beta-1 -3-N-Acetylglucosaminyltransferase 4
Beta3gn-T4
Database Links Reactome: R-HSA-2022854
Reactome: R-HSA-913709
Cellular Localisation Golgi Apparatus Membrane
Single-Pass Type Ii Membrane Protein
Alternative Antibody Names Anti-N-Acetyllactosaminide Beta-1 -3-N-Acetylglucosaminyltransferase 4 antibody
Anti-Udp-Glcnac -Betagal Beta-1 -3-N-Acetylglucosaminyltransferase 4 antibody
Anti-Bgnt-4 antibody
Anti-Beta-1 -3-Gn-T4 antibody
Anti-Beta-1 -3-N-Acetylglucosaminyltransferase 4 antibody
Anti-Beta3gn-T4 antibody
Anti-B3GNT4 antibody
Anti-UNQ1898 antibody
Anti-PRO4344 antibody

Information sourced from Uniprot.org

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