• Immunohistochemistry analysis of B3GNT3 in paraffin-embedded Rat ovary using B3GNT3 Rabbit polyclonal antibody (STJ119675) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of B3GNT3 in paraffin-embedded Human liver using B3GNT3 Rabbit polyclonal antibody (STJ119675) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates using B3GNT3 Rabbit polyclonal antibody (STJ119675) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-B3GNT3 antibody (120-240) (STJ119675)

SKU:
STJ119675

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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-B3GNT3 (120-240) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: B3GNT3
Gene ID: 10331
Uniprot ID: B3GN3_HUMAN
Immunogen Region: 120-240
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 120-240 of human B3GNT3 (NP_055071.2).
Immunogen Sequence: YVRRELLRRTWGRERKVRGL QLRLLFLVGTASNPHEARKV NRLLELEAQTHGDILQWDFH DSFFNLTLKQVLFLQWQETR CANASFVLNGDDDVFAHTDN MVFYLQDHDPGRHLFVGQLI Q
Tissue Specificity Expressed in colon, jejunum, stomach, esophagus, placenta and trachea.
Function Beta-1,3-N-acetylglucosaminyltransferase involved in the synthesis of poly-N-acetyllactosamine. Has activity for type 2 oligosaccharides. Also acts as a core1-1,3-N-acetylglucosaminyltransferase (Core1-beta3GlcNAcT) to form the 6-sulfo sialyl Lewis x on extended core1 O-glycans.
Protein Name N-Acetyllactosaminide Beta-1 -3-N-Acetylglucosaminyltransferase 3
Beta-1 -3-Galactosyl-O-Glycosyl-Glycoprotein Beta-1 -3-N-Acetylglucosaminyltransferase
Beta-1 -3-Galactosyltransferase 8
Beta-1 -3-Galtase 8
Beta3gal-T8
Beta3galt8
B3gal-T8
Beta-3-Gx-T8
Core 1 Extending Beta-1 -3-N-Acetylglucosaminyltransferase
Core1-Beta3glcnact
Transmembrane Protein 3
Udp-Gal -Beta-Glcnac Beta-1 -3-Galactosyltransferase 8
Udp-Glcnac -Betagal Beta-1 -3-N-Acetylglucosaminyltransferase 3
Bgnt-3
Beta-1 -3-Gn-T3
Beta-1 -3-N-Acetylglucosaminyltransferase 3
Beta3gn-T3
Udp-Galactose -Beta-N-Acetylglucosamine Beta-1 -3-Galactosyltransferase 8
Database Links Reactome: R-HSA-2022854
Reactome: R-HSA-913709
Cellular Localisation Golgi Apparatus Membrane
Single-Pass Type Ii Membrane Protein
Alternative Antibody Names Anti-N-Acetyllactosaminide Beta-1 -3-N-Acetylglucosaminyltransferase 3 antibody
Anti-Beta-1 -3-Galactosyl-O-Glycosyl-Glycoprotein Beta-1 -3-N-Acetylglucosaminyltransferase antibody
Anti-Beta-1 -3-Galactosyltransferase 8 antibody
Anti-Beta-1 -3-Galtase 8 antibody
Anti-Beta3gal-T8 antibody
Anti-Beta3galt8 antibody
Anti-B3gal-T8 antibody
Anti-Beta-3-Gx-T8 antibody
Anti-Core 1 Extending Beta-1 -3-N-Acetylglucosaminyltransferase antibody
Anti-Core1-Beta3glcnact antibody
Anti-Transmembrane Protein 3 antibody
Anti-Udp-Gal -Beta-Glcnac Beta-1 -3-Galactosyltransferase 8 antibody
Anti-Udp-Glcnac -Betagal Beta-1 -3-N-Acetylglucosaminyltransferase 3 antibody
Anti-Bgnt-3 antibody
Anti-Beta-1 -3-Gn-T3 antibody
Anti-Beta-1 -3-N-Acetylglucosaminyltransferase 3 antibody
Anti-Beta3gn-T3 antibody
Anti-Udp-Galactose -Beta-N-Acetylglucosamine Beta-1 -3-Galactosyltransferase 8 antibody
Anti-B3GNT3 antibody
Anti-B3GALT8 antibody
Anti-TMEM3 antibody
Anti-UNQ637 antibody
Anti-PRO1266 antibody

Information sourced from Uniprot.org

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