• Western blot analysis of extracts of various cell lines, using B3GALTL antibody (STJ111522) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 20s.

Anti-B3GALTL antibody (259-498) (STJ111522)

SKU:
STJ111522

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Host: Rabbit
Applications: WB
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-B3GALTL (259-498) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: B3GLCT
Gene ID: 145173
Uniprot ID: B3GLT_HUMAN
Immunogen Region: 259-498
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 259-498 of human B3GALTL (NP_919299.3).
Immunogen Sequence: LCRKPVKKKDIFVAVKTCKK FHGDRIPIVKQTWESQASLI EYYSDYTENSIPTVDLGIPN TDRGHCGKTFAILERFLNRS QDKTAWLVIVDDDTLISISR LQHLLSCYDSGEPVFLGERY GYGLGTGGYSYITGGGGMVF SREAVRRLLASKCRCYSNDA PDDMVLGMCFSGLGIPVTHS PLFHQARPVDYPKDYLSHQV PISFHKHWNIDPVKVYFTWL APSDEDKARQETQKGFREE
Tissue Specificity Widely expressed, with highest levels in testis and uterus.
Function O-glucosyltransferase that transfers glucose toward fucose with a beta-1,3 linkage. Specifically glucosylates O-linked fucosylglycan on TSP type-1 domains of proteins, thereby contributing to elongation of O-fucosylglycan.
Protein Name Beta-1 -3-Glucosyltransferase
Beta3glc-T
Beta 3-Glucosyltransferase
Beta-3-Glycosyltransferase-Like
Database Links Reactome: R-HSA-5083635
Reactome: R-HSA-5173214
Cellular Localisation Endoplasmic Reticulum Membrane
Single-Pass Type Ii Membrane Protein
Alternative Antibody Names Anti-Beta-1 -3-Glucosyltransferase antibody
Anti-Beta3glc-T antibody
Anti-Beta 3-Glucosyltransferase antibody
Anti-Beta-3-Glycosyltransferase-Like antibody
Anti-B3GLCT antibody
Anti-B3GALTL antibody
Anti-B3GTL antibody

Information sourced from Uniprot.org

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