• Western blot analysis of extracts of various cell lines, using ATP6V1E2 antibody (STJ114096) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-ATP6V1E2 antibody (90-180) (STJ114096)

SKU:
STJ114096

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-ATP6V1E2 (90-180) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ATP6V1E2
Gene ID: 90423
Uniprot ID: VATE2_HUMAN
Immunogen Region: 90-180
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 90-180 of human ATP6V1E2 (NP_542384.1).
Immunogen Sequence: LISDLLSEAKLRLSRIVEDP EVYQGLLDKLVLQGLLRLLE PVMIVRCRPQDLLLVEAAVQ KAIPEYMTISQKHVEVQIDK EAYLAVNAAGG
Tissue Specificity Testis specific.
Function Subunit of the V1 complex of vacuolar(H+)-ATPase (V-ATPase), a multisubunit enzyme composed of a peripheral complex (V1) that hydrolyzes ATP and a membrane integral complex (V0) that translocates protons. V-ATPase is responsible for acidifying and maintaining the pH of intracellular compartments and in some cell types, is targeted to the plasma membrane, where it is responsible for acidifying the extracellular environment.
Protein Name V-Type Proton Atpase Subunit E 2
V-Atpase Subunit E 2
Vacuolar Proton Pump Subunit E 2
Database Links Reactome: R-HSA-1222556
Reactome: R-HSA-77387
Reactome: R-HSA-917977
Reactome: R-HSA-9639288
Reactome: R-HSA-983712
Alternative Antibody Names Anti-V-Type Proton Atpase Subunit E 2 antibody
Anti-V-Atpase Subunit E 2 antibody
Anti-Vacuolar Proton Pump Subunit E 2 antibody
Anti-ATP6V1E2 antibody
Anti-ATP6E1 antibody
Anti-ATP6EL2 antibody
Anti-ATP6V1EL2 antibody

Information sourced from Uniprot.org

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