• Western blot analysis of lysates from 293 cells, treated with PMA 125ng/ml 30', using ATPase Antibody. The lane on the right is blocked with the synthesized peptide.
  • Immunofluorescence analysis of COS7 cells, using ATPase Antibody. The picture on the right is blocked with the synthesized peptide.
  • Immunohistochemistry analysis of paraffin-embedded human brain tissue, using ATPase Antibody. The picture on the right is blocked with the synthesized peptide.
  • Immunohistochemical analysis of paraffin-embedded Human kidney. 1, Antibody was diluted at 1:100 (4°C overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 30min).
  • Immunohistochemical analysis of paraffin-embedded Human kidney. 1, Antibody was diluted at 1:100 (4°C overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 30min).
  • Immunohistochemical analysis of paraffin-embedded Human kidney. 1, Antibody was diluted at 1:100 (4°C overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 30min).
  • Western blot analysis of 293 cells using Na+/K+-ATPase Alpha 1 Polyclonal Antibody diluted at 1:1000
  • Western blot analysis of various cells using Na+/K+-ATPase Alpha 1 Polyclonal Antibody diluted at 1:1000

Anti-ATP1A1 antibody (5-54) (STJ94338)

SKU:
STJ94338

Current Stock:
Host: Rabbit
Applications: WB/IHC/IF/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Sodium/potassium-transporting ATPase subunit alpha-1/K+ ATPase alpha-1 subunit (5-54) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-1:2000
IHC 1:100-1:300
IF 1:200-1:1000
ELISA 1:10000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ATP1A1
Gene ID: 476
Uniprot ID: AT1A1_HUMAN
Immunogen Region: 5-54
Specificity: Na+/K+-ATPase Alpha 1 Polyclonal Antibody detects endogenous levels of Na+/K+-ATPase Alpha 1 protein.
Immunogen: The antiserum was produced against synthesized peptide derived from human ATPase. AA range:5-54
Post Translational Modifications Phosphorylation on Tyr-10 modulates pumping activity. Phosphorylation of Ser-943 by PKA modulates the response of ATP1A1 to PKC. Dephosphorylation by protein phosphatase 2A (PP2A) following increases in intracellular sodium, leading to increase catalytic activity.
Function This is the catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of sodium and potassium ions across the plasma membrane. This action creates the electrochemical gradient of sodium and potassium ions, providing the energy for active transport of various nutrients.
Protein Name Sodium/Potassium-Transporting Atpase Subunit Alpha-1
Na(+/K(+ Atpase Alpha-1 Subunit
Sodium Pump Subunit Alpha-1
Database Links Reactome: R-HSA-5578775
Reactome: R-HSA-936837
Reactome: R-HSA-9679191
Cellular Localisation Basolateral Cell Membrane
Multi-Pass Membrane Protein
Cell Membrane
Sarcolemma
Cell Projection
Axon
Melanosome
Identified By Mass Spectrometry In Melanosome Fractions From Stage I To Stage Iv
Alternative Antibody Names Anti-Sodium/Potassium-Transporting Atpase Subunit Alpha-1 antibody
Anti-Na(+/K(+ Atpase Alpha-1 Subunit antibody
Anti-Sodium Pump Subunit Alpha-1 antibody
Anti-ATP1A1 antibody

Information sourced from Uniprot.org

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