• Western blot analysis of extracts of various cell lines, using ATP11B antibody (STJ114095) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.

Anti-ATP11B antibody (1-60) (STJ114095)

SKU:
STJ114095

£169.50 - £449.50
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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-ATP11B (1-60) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ATP11B
Gene ID: 23200
Uniprot ID: AT11B_HUMAN
Immunogen Region: 1-60
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-60 of human ATP11B (NP_055431.1).
Immunogen Sequence: MWRWIRQQLGFDPPHQSDTR TIYVANRFPQNGLYTPQKFI DNRIISSKYTVWNFVPKNLF
Function Catalytic component of a P4-ATPase flippase complex which catalyzes the hydrolysis of ATP coupled to the transport of aminophospholipids, phosphatidylserines (PS) and phosphatidylethanolamines (PE), from the outer to the inner leaflet of intracellular membranes. May contribute to the maintenance of membrane lipid asymmetry in endosome compartment.
Protein Name Phospholipid-Transporting Atpase If
Atpase Ir
Atpase Class Vi Type 11b
P4-Atpase Flippase Complex Alpha Subunit Atp11b
Database Links Reactome: R-HSA-6798695
Reactome: R-HSA-936837
Cellular Localisation Recycling Endosome Membrane
Multi-Pass Membrane Protein
Early Endosome
Endoplasmic Reticulum
Golgi Apparatus
Trans-Golgi Network
Exit From The Endoplasmic Reticulum Requires The Presence Of Tmem30a
But Not Tmem30b
In The Presence Of Tmem30a
Mainly Located In Recycling Endosomes
Alternative Antibody Names Anti-Phospholipid-Transporting Atpase If antibody
Anti-Atpase Ir antibody
Anti-Atpase Class Vi Type 11b antibody
Anti-P4-Atpase Flippase Complex Alpha Subunit Atp11b antibody
Anti-ATP11B antibody
Anti-ATPIF antibody
Anti-ATPIR antibody
Anti-KIAA0956 antibody

Information sourced from Uniprot.org

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