• Immunofluorescence analysis of SH-SY5Y cells using ATG9A Rabbit polyclonal antibody (STJ117815) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of HepG2 cells using ATG9A Rabbit polyclonal antibody (STJ117815) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Western blot analysis of extracts of U-251MG cells, using ATG9A antibody (STJ117815) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-ATG9A antibody (700-800) (STJ117815)

SKU:
STJ117815

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-ATG9A (700-800) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ATG9A
Gene ID: 79065
Uniprot ID: ATG9A_HUMAN
Immunogen Region: 700-800
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 700-800 of human ATG9A (NP_076990.4).
Immunogen Sequence: EYASTEMSLHALYMHQLHKQ QAQAEPERHVWHRRESDESG ESAPDEGGEGARAPQSIPRS ASYPCAAPRPGAPETTALHG GFQRRYGGITDPGTVPRVPS H
Function Phospholipid scramblase involved in autophagy by mediating autophagosomal membrane expansion. Cycles between the preautophagosomal structure/phagophore assembly site (PAS) and the cytoplasmic vesicle pool and supplies membrane for the growing autophagosome. Lipid scramblase activity plays a key role in preautophagosomal structure/phagophore assembly by distributing the phospholipids that arrive through ATG2 (ATG2A or ATG2B) from the cytoplasmic to the luminal leaflet of the bilayer, thereby driving autophagosomal membrane expansion. Also required to supply phosphatidylinositol 4-phosphate to the autophagosome initiation site by recruiting the phosphatidylinositol 4-kinase beta (PI4KB) in a process dependent on ARFIP2, but not ARFIP1. In addition to autophagy, also plays a role in necrotic cell death.
Protein Name Autophagy-Related Protein 9a
Apg9-Like 1
Matg9
Database Links Reactome: R-HSA-1632852
Cellular Localisation Preautophagosomal Structure Membrane
Multi-Pass Membrane Protein
Cytoplasmic Vesicle
Autophagosome Membrane
Golgi Apparatus
Trans-Golgi Network Membrane
Late Endosome Membrane
Recycling Endosome Membrane
Endoplasmic Reticulum Membrane
Mitochondrion Membrane
Mainly Localizes To The Trans-Golgi Network (Tgn) And The Endosomal System
Cycles Between Them Though Vesicle Trafficking
Export From The Tgn To Promote Formation Of Autophagosomes Is Mediated By The Ap-4 Complex
Under Amino Acid Starvation Or Rapamycin Treatment
Redistributes To Preautophagosomal Structure/Phagophore Assembly Site (Pas)
The Starvation-Induced Redistribution Depends On Ulk1
Atg13
As Well As Sh3glb1
Upon Autophagy Induction
A Small Portion Transiently Localizes To The Autophagic Membranes
Recruited To Damaged Mitochondria During Mitophagy In A Rimoc1-Dependent Manner
Alternative Antibody Names Anti-Autophagy-Related Protein 9a antibody
Anti-Apg9-Like 1 antibody
Anti-Matg9 antibody
Anti-ATG9A antibody
Anti-APG9L1 antibody

Information sourced from Uniprot.org

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