• Western blot analysis of extracts of various cell lines, using ATG4B antibody (STJ11104328) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 60s.

Anti-ATG4B antibody (1-393) (STJ11104328)

SKU:
STJ11104328

Current Stock:
Host: Rabbit
Applications: WB
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Cysteine Protease Atg4b (1-393) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ATG4B
Gene ID: 23192
Uniprot ID: ATG4B_HUMAN
Immunogen Region: 1-393
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-393 of human ATG4B (NP_037457.3).
Immunogen Sequence: MDAATLTYDTLRFAEFEDFP ETSEPVWILGRKYSIFTEKD EILSDVASRLWFTYRKNFPA IGGTGPTSDTGWGCMLRCGQ MIFAQALVCRHLGRDWRWTQ RKRQPDSYFSVLNAFIDRKD SYYSIHQIAQMGVGEGKSIG QWYGPNTVAQVLKKLAVFDT WSSLAVHIAMDNTVVMEEIR RLCRTSVPCAGATAFPADSD RHCNGFPAGAEVTNRPSPWR PLVLLIPLRLGLTDINEAY
Post Translational Modifications Phosphorylation at Ser-383 and Ser-392 promotes autophagy by increasing protein delipidation activity without affecting proteolytic activation of ATG8 proteins. Phosphorylation at Ser-316 by ULK1 inhibits autophagy by decreasing both proteolytic activation and delipidation activities. Phosphorylation at Ser-316 is dephosphorylated by protein phosphatase 2A (PP2A). Phosphorylation at Ser-34 by AKT2 promotes its hydrolase activity, leading to increased proteolytic activation and delipidation of ATG8 family proteins. Phosphorylation at Ser-34 by AKT1 promotes mitochondrial localization and inhibition of the F1F0-ATP synthase activity, leading to elevation of mitochondrial reactive oxygen species (ROS). Ubiquitinated by RNF5, leading to its degradation by the proteasome. S-nitrosylation at Cys-189 and Cys-292 in response to high glucose decreases both proteolytic activation and delipidation activities. O-glycosylated by OGT, leading to increase protease activity, thereby promoting the proteolytic activation of ATG8 family proteins. Forms reversible intrachain disulfide bonds in response to oxidative stress. Forms interchain disulfide bonds, leading to formation of homooligomers in response to oxidation.
Function Cysteine protease that plays a key role in autophagy by mediating both proteolytic activation and delipidation of ATG8 family proteins. Required for canonical autophagy (macroautophagy), non-canonical autophagy as well as for mitophagy. The protease activity is required for proteolytic activation of ATG8 family proteins: cleaves the C-terminal amino acid of ATG8 proteins MAP1LC3A, MAP1LC3B, MAP1LC3C, GABARAPL1, GABARAPL2 and GABARAP, to reveal a C-terminal glycine. Exposure of the glycine at the C-terminus is essential for ATG8 proteins conjugation to phosphatidylethanolamine (PE) and insertion to membranes, which is necessary for autophagy. Protease activity is also required to counteract formation of high-molecular weight conjugates of ATG8 proteins (ATG8ylation): acts as a deubiquitinating-like enzyme that removes ATG8 conjugated to other proteins, such as ATG3. In addition to the protease activity, also mediates delipidation of ATG8 family proteins. Catalyzes delipidation of PE-conjugated forms of ATG8 proteins during macroautophagy. Also involved in non-canonical autophagy, a parallel pathway involving conjugation of ATG8 proteins to single membranes at endolysosomal compartments, by catalyzing delipidation of ATG8 proteins conjugated to phosphatidylserine (PS). Compared to other members of the family (ATG4A, ATG4C or ATG4C), constitutes the major protein for proteolytic activation of ATG8 proteins, while it displays weaker delipidation activity than other ATG4 paralogs. Involved in phagophore growth during mitophagy independently of its protease activity and of ATG8 proteins: acts by regulating ATG9A trafficking to mitochondria and promoting phagophore-endoplasmic reticulum contacts during the lipid transfer phase of mitophagy.
Protein Name Cysteine Protease Atg4b
Aut-Like 1 Cysteine Endopeptidase
Autophagy-Related Cysteine Endopeptidase 1
Autophagin-1
Autophagy-Related Protein 4 Homolog B
Hsapg4b
Hapg4b
Database Links Reactome: R-HSA-1632852
Cellular Localisation Cytoplasm
Cytosol
Cytoplasmic Vesicle
Autophagosome
Endoplasmic Reticulum
Mitochondrion
Mainly Localizes To The Cytoplasm
Including Cytosol
A Samll Potion Localizes To Mitochondria
Phosphorylation At Ser-34 Promotes Localization To Mitochondria
Alternative Antibody Names Anti-Cysteine Protease Atg4b antibody
Anti-Aut-Like 1 Cysteine Endopeptidase antibody
Anti-Autophagy-Related Cysteine Endopeptidase 1 antibody
Anti-Autophagin-1 antibody
Anti-Autophagy-Related Protein 4 Homolog B antibody
Anti-Hsapg4b antibody
Anti-Hapg4b antibody
Anti-ATG4B antibody
Anti-APG4B antibody
Anti-AUTL1 antibody
Anti-KIAA0943 antibody

Information sourced from Uniprot.org

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