• Western blot analysis of extracts of various cell lines, using ARFGAP3 antibody (STJ110503) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-ARFGAP3 antibody (217-516) (STJ110503)

SKU:
STJ110503

£169.50 - £449.50
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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-ARFGAP3 (217-516) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ARFGAP3
Gene ID: 26286
Uniprot ID: ARFG3_HUMAN
Immunogen Region: 217-516
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 217-516 of human ARFGAP3 (NP_055385.3).
Immunogen Sequence: KPNQAKKGLGAKKGSLGAQK LANTCFNEIEKQAQAADKMK EQEDLAKVVSKEESIVSSLR LAYKDLEIQMKKDEKMNISG KKNVDSDRLGMGFGNCRSVI SHSVTSDMQTIEQESPIMAK PRKKYNDDSDDSYFTSSSSY FDEPVELRSSSFSSWDDSSD SYWKKETSKDTETVLKTTGY SDRPTARRKPDYEPVENTDE AQKKFGNVKAISSDMYFGRQ SQADYETRARLERLSASSS
Tissue Specificity Widely expressed. Highest expression in endocrine glands (pancreas, pituitary gland, salivary gland, and prostate) and testis with a much higher expression in the testis than in the ovary.
Function GTPase-activating protein (GAP) for ADP ribosylation factor 1 (ARF1). Hydrolysis of ARF1-bound GTP may lead to dissociation of coatomer from Golgi-derived membranes to allow fusion with target membranes.
Protein Name Adp-Ribosylation Factor Gtpase-Activating Protein 3
Arf Gap 3
Database Links Reactome: R-HSA-6807878
Reactome: R-HSA-6811434
Reactome: R-HSA-9013148
Reactome: R-HSA-9013408
Cellular Localisation Cytoplasm
Golgi Apparatus Membrane
Peripheral Membrane Protein
Cytoplasmic Side
Also Found On Peripheral Punctate Structures Likely To Be Endoplasmic Reticulum-Golgi Intermediate Compartment
Alternative Antibody Names Anti-Adp-Ribosylation Factor Gtpase-Activating Protein 3 antibody
Anti-Arf Gap 3 antibody
Anti-ARFGAP3 antibody
Anti-ARFGAP1 antibody

Information sourced from Uniprot.org

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