| Host: |
Rabbit |
| Applications: |
WB/ELISA |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-E3 ubiquitin-protein ligase RNF14 (361-410 aa) is suitable for use in Western Blot and ELISA research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Purification: |
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration: |
1 mg/mL |
| Dilution Range: |
WB 1:500-1:2000ELISA 1:40000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
RNF14 |
| Gene ID: |
9604 |
| Uniprot ID: |
RNF14_HUMAN |
| Immunogen Region: |
361-410 aa |
| Specificity: |
ARA54 Polyclonal Antibody detects endogenous levels of ARA54 protein. |
| Immunogen: |
The antiserum was produced against synthesized peptide derived from the human RNF14 at the amino acid range 361-410 |
| Post Translational Modifications | RING-type zinc finger-dependent and UBE2E2-dependent autoubiquitination. |
| Function | E3 ubiquitin-protein ligase that plays a key role in the RNF14-RNF25 translation quality control pathway, a pathway that takes place when a ribosome has stalled during translation, and which promotes ubiquitination and degradation of translation factors on stalled ribosomes. Recruited to stalled ribosomes by the ribosome collision sensor GCN1 and mediates ubiquitination of EEF1A1/eEF1A, leading to its degradation. In addition to EEF1A1/eEF1A, RNF14 catalyzes ubiquitination of other translation factors on stalled ribosomes: mediates ubiquitination and degradation of ETF1/eRF1 and ubiquitination of ribosomal proteins RPL0, RPL1, RPL12, RPS13 and RPS17. Independently of its function in the response to stalled ribosomes, acts as a regulator of transcription in Wnt signaling via its interaction with TCF transcription factors (TCF7/TCF1, TCF7L1/TCF3 and TCF7L2/TCF4). May also play a role as a coactivator for androgen- and, to a lesser extent, progesterone-dependent transcription. |
| Protein Name | E3 Ubiquitin-Protein Ligase Rnf14Androgen Receptor-Associated Protein 54Hfb30Ring Finger Protein 14 |
| Database Links | Reactome: R-HSA-983168 |
| Cellular Localisation | CytoplasmNucleus |
| Alternative Antibody Names | Anti-E3 Ubiquitin-Protein Ligase Rnf14 antibodyAnti-Androgen Receptor-Associated Protein 54 antibodyAnti-Hfb30 antibodyAnti-Ring Finger Protein 14 antibodyAnti-RNF14 antibodyAnti-ARA54 antibodyAnti-HRIHFB2038 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
