• Western blot analysis of various lysates using APPL2 Rabbit polyclonal antibody (STJ116800) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-APPL2 antibody (1-300) (STJ116800)

SKU:
STJ116800

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-APPL2 (1-300) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: APPL2
Gene ID: 55198
Uniprot ID: DP13B_HUMAN
Immunogen Region: 1-300
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human APPL2 (NP_060641.2).
Immunogen Sequence: MPAVDKLLLEEALQDSPQTR SLLSVFEEDAGTLTDYTNQL LQAMQRVYGAQNEMCLATQQ LSKQLLAYEKQNFALGKGDE EVISTLHYFSKVVDELNLLH TELAKQLADTMVLPIIQFRE KDLTEVSTLKDLFGLASNEH DLSMAKYSRLPKKKENEKVK TEVGKEVAAARRKQHLSSLQ YYCALNALQYRKQMAMMEPM IGFAHGQINFFKKGAEMFSK RMDSFLSSVADMVQSIQVE
Tissue Specificity High levels in brain, heart, kidney and skeletal muscle.
Function Multifunctional adapter protein that binds to various membrane receptors, nuclear factors and signaling proteins to regulate many processes, such as cell proliferation, immune response, endosomal trafficking and cell metabolism. Regulates signaling pathway leading to cell proliferation through interaction with RAB5A and subunits of the NuRD/MeCP1 complex. Plays a role in immune response by modulating phagocytosis, inflammatory and innate immune responses. In macrophages, enhances Fc-gamma receptor-mediated phagocytosis through interaction with RAB31 leading to activation of PI3K/Akt signaling. In response to LPS, modulates inflammatory responses by playing a key role on the regulation of TLR4 signaling and in the nuclear translocation of RELA/NF-kappa-B p65 and the secretion of pro- and anti-inflammatory cytokines. Also functions as a negative regulator of innate immune response via inhibition of AKT1 signaling pathway by forming a complex with APPL1 and PIK3R1. Plays a role in endosomal trafficking of TGFBR1 from the endosomes to the nucleus. Plays a role in cell metabolism by regulating adiponecting ans insulin signaling pathways and adaptative thermogenesis. In muscle, negatively regulates adiponectin-simulated glucose uptake and fatty acid oxidation by inhibiting adiponectin signaling pathway through APPL1 sequestration thereby antagonizing APPL1 action. In muscles, negativeliy regulates insulin-induced plasma membrane recruitment of GLUT4 and glucose uptake through interaction with TBC1D1. Plays a role in cold and diet-induced adaptive thermogenesis by activating ventromedial hypothalamus (VMH) neurons throught AMPK inhibition which enhances sympathetic outflow to subcutaneous white adipose tissue (sWAT), sWAT beiging and cold tolerance. Also plays a role in other signaling pathways namely Wnt/beta-catenin, HGF and glucocorticoid receptor signaling. Positive regulator of beta-catenin/TCF-dependent transcription through direct interaction with RUVBL2/reptin resulting in the relief of RUVBL2-mediated repression of beta-catenin/TCF target genes by modulating the interactions within the beta-catenin-reptin-HDAC complex. May affect adult neurogenesis in hippocampus and olfactory system via regulating the sensitivity of glucocorticoid receptor. Required for fibroblast migration through HGF cell signaling.
Protein Name Dcc-Interacting Protein 13-Beta
Dip13-Beta
Adapter Protein Containing Ph Domain - Ptb Domain And Leucine Zipper Motif 2
Cellular Localisation Early Endosome Membrane
Peripheral Membrane Protein
Nucleus
Cell Membrane
Endosome Membrane
Cytoplasm
Cytoplasmic Vesicle
Phagosome
Cell Projection
Ruffle
Ruffle Membrane
Phagosome Membrane
Early Endosomal Membrane-Bound And Nuclear
Translocated Into The Nucleus Upon Release From Endosomal Membranes Following Internalization Of Egf
Associates Dynamically With Cytoplasmic Membrane Structures That Undergo Changes In Shape
Movement
Fusion And Fission Events
Pi(4
5)P2 Levels Are Important For Membrane Association Of Appl2
Absent Of Endosome In Macrophage
Colocalized With Rab31 At Early-Stage Phagosome
Localized On Macropinosomes In Lps-Activated Macrophages
Associated With Membrane Domains In Contact With Pathogens And Pathogen-Derived Ligands Like Lps
First Recruited To The Ruffles
And Accumulates On Macropinosomes
Alternative Antibody Names Anti-Dcc-Interacting Protein 13-Beta antibody
Anti-Dip13-Beta antibody
Anti-Adapter Protein Containing Ph Domain - Ptb Domain And Leucine Zipper Motif 2 antibody
Anti-APPL2 antibody
Anti-DIP13B antibody

Information sourced from Uniprot.org

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