• Western blot analysis of extracts of various cell lines, using APOL2 antibody (STJ114094) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-APOL2 antibody (170-270) (STJ114094)

SKU:
STJ114094

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Host: Rabbit
Applications: WB
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-APOL2 (170-270) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: APOL2
Gene ID: 23780
Uniprot ID: APOL2_HUMAN
Immunogen Region: 170-270
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 170-270 of human APOL2 (NP_663612.2).
Immunogen Sequence: ELVNKLRARAQARNLDQSGT NVAKVMKEFVGGNTPNVLTL VDNWYQVTQGIGRNIRAIRR ARANPQLGAYAPPPHVIGRI SAEGGEQVERVVEGPAQAMS R
Tissue Specificity Widely expressed.the highest levels are found in lung, thymus, pancreas, placenta, adult brain and prostate.also detected in spleen, liver, kidney, colon, small intestine, uterus, spinal cord, adrenal gland, salivary gland, trachea, mammary gland, skeletal muscle, testis and fetal brain and liver.
Function May affect the movement of lipids in the cytoplasm or allow the binding of lipids to organelles.
Protein Name Apolipoprotein L2
Apolipoprotein L-Ii
Apol-Ii
Cellular Localisation Cytoplasm
Alternative Antibody Names Anti-Apolipoprotein L2 antibody
Anti-Apolipoprotein L-Ii antibody
Anti-Apol-Ii antibody
Anti-APOL2 antibody

Information sourced from Uniprot.org

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