• Immunohistochemistry analysis of paraffin-embedded human placenta tissue, using APOBEC3D/F Antibody. The picture on the right is blocked with the synthesized peptide.
  • Western blot analysis of lysates from COS7 and MCF-7 cells, using APOBEC3D/F Antibody. The lane on the right is blocked with the synthesized peptide.
  • Western blot analysis of various cells using APOBEC3D/F Polyclonal Antibody

Anti-APOBEC3D/F antibody (232-281 aa) (STJ91636)

SKU:
STJ91636

£46.00 - £226.00
Current Stock:
Host: Rabbit
Applications: WB/IHC/IF/ELISA
Reactivity: Human/Monkey
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-DNA dC->dU-editing enzyme APOBEC-3D and DNA dC->dU-editing enzyme APOBEC-3F (232-281 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-1:2000
IHC 1:100-1:300
ELISA 1:40000
IF 1:50-200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: APOBEC3F
APOBEC3D
Gene ID: 200316
NA
Uniprot ID: ABC3F_HUMAN
ABC3D_HUMAN
Immunogen Region: 232-281 aa
Specificity: APOBEC3D/F Polyclonal Antibody detects endogenous levels of APOBEC3D/F protein.
Immunogen: The antiserum was produced against synthesized peptide derived from the human APOBEC3D/F at the amino acid range 232-281
Function DNA deaminase (cytidine deaminase) which acts as an inhibitor of retrovirus replication and retrotransposon mobility via deaminase-dependent and -independent mechanisms. Exhibits antiviral activity against Vif-deficient HIV-1. After the penetration of retroviral nucleocapsids into target cells of infection and the initiation of reverse transcription, it can induce the conversion of cytosine to uracil in the minus-sense single-strand viral DNA, leading to G-to-A hypermutations in the subsequent plus-strand viral DNA. The resultant detrimental levels of mutations in the proviral genome, along with a deamination-independent mechanism that works prior to the proviral integration, together exert efficient antiretroviral effects in infected target cells. Selectively targets single-stranded DNA and does not deaminate double-stranded DNA or single- or double-stranded RNA. Exhibits antiviral activity also against hepatitis B virus (HBV), equine infectious anemia virus (EIAV), xenotropic MuLV-related virus (XMRV) and simian foamy virus (SFV) and may inhibit the mobility of LTR and non-LTR retrotransposons. May also play a role in the epigenetic regulation of gene expression through the process of active DNA demethylation.
Protein Name Dna Dc->Du-Editing Enzyme Apobec-3f
Apolipoprotein B Mrna-Editing Enzyme Catalytic Polypeptide-Like 3f
A3f
Cellular Localisation Cytoplasm
P-Body
Alternative Antibody Names Anti-Dna Dc->Du-Editing Enzyme Apobec-3f antibody
Anti-Apolipoprotein B Mrna-Editing Enzyme Catalytic Polypeptide-Like 3f antibody
Anti-A3f antibody
Anti-APOBEC3F antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance