| Host: |
Rabbit |
| Applications: |
WB/IHC |
| Reactivity: |
Human/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-ANKRD27 (560-710) is suitable for use in Western Blot and Immunohistochemistry research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:2000IHC-P 1:50-1:200 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
ANKRD27 |
| Gene ID: |
84079 |
| Uniprot ID: |
ANR27_HUMAN |
| Immunogen Region: |
560-710 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 560-710 of human ANKRD27 (NP_115515.2). |
| Immunogen Sequence: |
IGNEKGDTPLHIAARWGYQG VIETLLQNGASTEIQNRLKE TPLKCALNSKILSVMEAYHL SFERRQKSSEAPVQSPQRSV DSISQESSTSSFSSMSASSR QEETKKDYREVEKLLRAVAD GDLEMVRYLLEWTEEDLEDA EDTVSAADPEF |
| Function | May be a guanine exchange factor (GEF) for Rab21, Rab32 and Rab38 and regulate endosome dynamics. May regulate the participation of VAMP7 in membrane fusion events.in vitro inhibits VAMP7-mediated SNARE complex formation by trapping VAMP7 in a closed, fusogenically inactive conformation. Involved in peripheral melanosomal distribution of TYRP1 in melanocytes.the function, which probably is implicating vesicle-trafficking, includes cooperation with Rab32, Rab38 and VAMP7. Involved in the regulation of neurite growth.the function seems to require its GEF activity, probably towards Rab21, and VAMP7 but not Rab32/38. Proposed to be involved in Golgi sorting of VAMP7 and transport of VAMP7 vesicles to the cell surface.the function seems to implicate kinesin heavy chain isoform 5 proteins, GOLGA4, RAB21 and MACF1. Required for the colocalization of VAMP7 and Rab21, probably on TGN sites. Involved in GLUT1 endosome-to-plasma membrane trafficking.the function is dependent of association with VPS29. Regulates the proper trafficking of melanogenic enzymes TYR, TYRP1 and DCT/TYRP2 to melanosomes in melanocytes. |
| Protein Name | Ankyrin Repeat Domain-Containing Protein 27Vps9 Domain-Containing Protein |
| Database Links | Reactome: R-HSA-8876198 |
| Cellular Localisation | Early EndosomeLate EndosomeCytoplasmic Vesicle MembraneLysosomeCell MembraneMelanosomeColocalizes With Vamp7 In Transport Vesicles In The Shaft Of Hippocampal Neurons |
| Alternative Antibody Names | Anti-Ankyrin Repeat Domain-Containing Protein 27 antibodyAnti-Vps9 Domain-Containing Protein antibodyAnti-ANKRD27 antibodyAnti-PP12899 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
