| Host: |
Rabbit |
| Applications: |
WB/IF |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-ALOX15B (1-351) is suitable for use in Western Blot and Immunofluorescence research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.05% Proclin300, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:100-1:500IF/ICC 1:50-1:200 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
ALOX15B |
| Gene ID: |
247 |
| Uniprot ID: |
LX15B_HUMAN |
| Immunogen Region: |
1-351 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 1-351 of human ALOX15B (NP_001132.2). |
| Immunogen Sequence: |
MAEFRVRVSTGEAFGAGTWD KVSVSIVGTRGESPPLPLDN LGKEFTAGAEEDFQVTLPED VGRVLLLRVHKAPPVLPLLG PLAPDAWFCRWFQLTPPRGG HLLFPCYQWLEGAGTLVLQE GTAKVSWADHHPVLQQQRQE ELQARQEMYQWKAYNPGWPH CLDEKTVEDLELNIKYSTAK NANFYLQAGSAFAEMKIKGL |
| Tissue Specificity | Expressed in hair, prostate, lung, ovary, lymph node, spinal cord and cornea. |
| Function | Isoform A: Non-heme iron-containing dioxygenase that catalyzes the stereo-specific peroxidation of free and esterified polyunsaturated fatty acids (PUFAs) generating a spectrum of bioactive lipid mediators (Probable). It inserts peroxyl groups at C15 of arachidonate ((5Z,8Z,11Z,14Z)-eicosatetraenoate) producing (15S)-hydroperoxyeicosatetraenoate/(15S)-HPETE (Probable). Also peroxidizes linoleate ((9Z,12Z)-octadecadienoate) to 13-hydroperoxyoctadecadienoate/13-HPODE (Probable). Oxygenates arachidonyl derivatives such as 2-arachidonoylglycerol (2-AG) leading to the production and extracellular release of 15-hydroxyeicosatetraenoyl glycerol (15-HETE-G) that acts as a peroxisome proliferator-activated receptor alpha agonist. Has the ability to efficiently class-switch ALOX5 pro-inflammatory mediators into anti-inflammatory intermediates. Participates in the sequential oxidations of DHA ((4Z,7Z,10Z,13Z,16Z,19Z)-docosahexaenoate) to generate specialized pro-resolving mediators (SPMs) resolvin D5 ((7S,17S)-diHPDHA), which can actively down-regulate the immune response and have anti-aggregation properties with platelets. In addition to free PUFAs hydrolyzed from phospholipids, it directly oxidizes PUFAs esterified to membrane-bound phospholipids. Has no detectable 8S-lipoxygenase activity on arachidonate but reacts with (8S)-HPETE to produce (8S,15S)-diHPETE (Probable). May regulate progression through the cell cycle and cell proliferation. May also regulate cytokine secretion by macrophages and therefore play a role in the immune response. May also regulate macrophage differentiation into proatherogenic foam cells. Isoform B: Does not convert arachidonic acid to 15S-hydroperoxyeicosatetraenoic acid/(15S)-HPETE. |
| Protein Name | Polyunsaturated Fatty Acid Lipoxygenase Alox15b15-Lipoxygenase 215-Lox-2Arachidonate 15-Lipoxygenase B15-Lox-BArachidonate 15-Lipoxygenase Type IiLinoleate 13-Lipoxygenase 15-Lob |
| Database Links | Reactome: R-HSA-2142770 |
| Cellular Localisation | Isoform A: NucleusOther Isoforms Are Excluded From The NucleusCytoplasmCytosolCell MembraneCytoskeletonMembranePeripheral Membrane ProteinCell JunctionAdherens JunctionFocal AdhesionPredominantly CytosolicBecomes Enriched At Membranes Upon Calcium Binding |
| Alternative Antibody Names | Anti-Polyunsaturated Fatty Acid Lipoxygenase Alox15b antibodyAnti-15-Lipoxygenase 2 antibodyAnti-15-Lox-2 antibodyAnti-Arachidonate 15-Lipoxygenase B antibodyAnti-15-Lox-B antibodyAnti-Arachidonate 15-Lipoxygenase Type Ii antibodyAnti-Linoleate 13-Lipoxygenase 15-Lob antibodyAnti-ALOX15B antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
