• Western blot analysis of extracts of various cell lines, using ALKBH1 antibody (STJ27996) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 30s.
  • Immunoprecipitation analysis of 200ug extracts of 293T cells using 1ug ALKBH1 antibody (STJ27996). Western blot was performed from the immunoprecipitate using ALKBH1 antibody (STJ27996) at a dilition of 1:1000.

Anti-ALKBH1 antibody (110-389) (STJ27996)

SKU:
STJ27996

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Host: Rabbit
Applications: WB/IP
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-ALKBH1 (110-389) is suitable for use in Western Blot and Immunoprecipitation research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IP 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ALKBH1
Gene ID: 8846
Uniprot ID: ALKB1_HUMAN
Immunogen Region: 110-389
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 110-389 of human ALKBH1 (NP_006011.2).
Immunogen Sequence: YQWHWVKQCLKLYSQKPNVC NLDKHMSKEETQDLWEQSKE FLRYKEATKRRPRSLLEKLR WVTVGYHYNWDSKKYSADHY TPFPSDLGFLSEQVAAACGF EDFRAEAGILNYYRLDSTLG IHVDRSELDHSKPLLSFSFG QSAIFLLGGLQRDEAPTAMF MHSGDIMIMSGFSRLLNHAV PRVLPNPEGEGLPHCLEAPL PAVLPRDSMVEPCSMEDWQV CASYLKTARVNMTVRQVLA
Tissue Specificity Ubiquitous.
Function Dioxygenase that acts as on nucleic acids, such as DNA and tRNA. Requires molecular oxygen, alpha-ketoglutarate and iron. A number of activities have been described for this dioxygenase, but recent results suggest that it mainly acts as on tRNAs and mediates their demethylation or oxidation depending on the context and subcellular compartment. Mainly acts as a tRNA demethylase by removing N(1)-methyladenine from various tRNAs, with a preference for N(1)-methyladenine at position 58 (m1A58) present on a stem loop structure of tRNAs. Acts as a regulator of translation initiation and elongation in response to glucose deprivation: regulates both translation initiation, by mediating demethylation of tRNA(Met), and translation elongation, N(1)-methyladenine-containing tRNAs being preferentially recruited to polysomes to promote translation elongation. In mitochondrion, specifically interacts with mt-tRNA(Met) and mediates oxidation of mt-tRNA(Met) methylated at cytosine(34) to form 5-formylcytosine (f(5)c) at this position. mt-tRNA(Met) containing the f(5)c modification at the wobble position enables recognition of the AUA codon in addition to the AUG codon, expanding codon recognition in mitochondrial translation. Specifically demethylates DNA methylated on the 6th position of adenine (N(6)-methyladenosine) DNA. N(6)-methyladenosine (m6A) DNA is present at some L1 elements in embryonic stem cells and probably promotes their silencing. Demethylates mRNAs containing N(3)-methylcytidine modification. Also able to repair alkylated single-stranded DNA by oxidative demethylation, but with low activity. Also has DNA lyase activity and introduces double-stranded breaks at abasic sites: cleaves both single-stranded DNA and double-stranded DNA at abasic sites, with the greatest activity towards double-stranded DNA with two abasic sites. DNA lyase activity does not require alpha-ketboglutarate and iron and leads to the formation of an irreversible covalent protein-DNA adduct with the 5' DNA product. DNA lyase activity is not required during base excision repair and class switch recombination of the immunoglobulin heavy chain during B lymphocyte activation. May play a role in placental trophoblast lineage differentiation.
Protein Name Nucleic Acid Dioxygenase Alkbh1
Alkylated Dna Repair Protein Alkb Homolog 1
Alpha-Ketoglutarate-Dependent Dioxygenase Abh1
Dna 6ma Demethylase
Dna N6-Methyl Adenine Demethylase Alkbh1
Dna Lyase Abh1
Dna Oxidative Demethylase Alkbh1
Mrna N(3-Methylcytidine Demethylase
Cellular Localisation Nucleus
Mitochondrion
Mainly Localizes In Euchromatin
Largely Excluded From Heterochromatin And Nucleoli
Alternative Antibody Names Anti-Nucleic Acid Dioxygenase Alkbh1 antibody
Anti-Alkylated Dna Repair Protein Alkb Homolog 1 antibody
Anti-Alpha-Ketoglutarate-Dependent Dioxygenase Abh1 antibody
Anti-Dna 6ma Demethylase antibody
Anti-Dna N6-Methyl Adenine Demethylase Alkbh1 antibody
Anti-Dna Lyase Abh1 antibody
Anti-Dna Oxidative Demethylase Alkbh1 antibody
Anti-Mrna N(3-Methylcytidine Demethylase antibody
Anti-ALKBH1 antibody
Anti-ABH antibody
Anti-ABH1 antibody
Anti-ALKBH antibody

Information sourced from Uniprot.org

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