• Immunohistochemistry analysis of paraffin-embedded rat brain using AKTIP antibody (STJ27995) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of various cell lines, using AKTIP antibody (STJ27995) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 40s.

Anti-AKTIP antibody (1-292) (STJ27995)

SKU:
STJ27995

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-AKTIP (1-292) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: AKTIP
Gene ID: 64400
Uniprot ID: AKTIP_HUMAN
Immunogen Region: 1-292
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-292 of human AKTIP (NP_071921.1).
Immunogen Sequence: MNPFWSMSTSSVRKRSEGEE KTLTGDVKTSPPRTAPKKQL PSIPKNALPITKPTSPAPAA QSTNGTHASYGPFYLEYSLL AEFTLVVKQKLPGVYVQPSY RSALMWFGVIFIRHGLYQDG VFKFTVYIPDNYPDGDCPRL VFDIPVFHPLVDPTSGELDV KRAFAKWRRNHNHIWQVLMY ARRVFYKIDTASPLNPEAAV LYEKDIQLFKSKVVDSVKVC TARLFDQPKIEDPYAISFS
Function Component of the FTS/Hook/FHIP complex (FHF complex). The FHF complex may function to promote vesicle trafficking and/or fusion via the homotypic vesicular protein sorting complex (the HOPS complex). Regulates apoptosis by enhancing phosphorylation and activation of AKT1. Increases release of TNFSF6 via the AKT1/GSK3B/NFATC1 signaling cascade. FHF complex promotes the distribution of AP-4 complex to the perinuclear area of the cell.
Protein Name Akt-Interacting Protein
Ft1
Fused Toes Protein Homolog
Cellular Localisation Cytoplasm
Cell Membrane
Peripheral Membrane Protein
Alternative Antibody Names Anti-Akt-Interacting Protein antibody
Anti-Ft1 antibody
Anti-Fused Toes Protein Homolog antibody
Anti-AKTIP antibody
Anti-FTS antibody

Information sourced from Uniprot.org

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