| Host: | Rabbit |
| Applications: | WB/IF/ICC/ELISA |
| Reactivity: | Human/Mouse |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Monoclonal |
| Clone ID : | S2MR |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH 7.3. |
| Purification: | Affinity purification |
| Concentration: | Lot specific |
| Dilution Range: | WB:1:1000-1:6000 IF/ICC:1:50-1:200 ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | AKR1C3 |
| Gene ID: | 8644 |
| Uniprot ID: | AK1C3_HUMAN |
| Immunogen Region: | 200-300 |
| Specificity: | A synthetic peptide corresponding to a sequence within amino acids 200-300 of human AKR1C3 (P42330). |
| Immunogen Sequence: | SKLLDFCKSKDIVLVAYSAL GSQRDKRWVDPNSPVLLEDP VLCALAKKHKRTPALIALRY QLQRGVVVLAKSYNEQRIRQ NVQVFEFQLTAEDMKAIDGL D |
| Tissue Specificity | Expressed in many tissues including adrenal gland, brain, kidney, liver, lung, mammary gland, placenta, small intestine, colon, spleen, prostate and testis. High expression in prostate and mammary gland. In the prostate, higher levels in epithelial cells than in stromal cells. In the brain, expressed in medulla, spinal cord, frontotemporal lobes, thalamus, subthalamic nuclei and amygdala. Weaker expression in the hippocampus, substantia nigra and caudate. |
| Function | Cytosolic aldo-keto reductase that catalyzes the NADH and NADPH-dependent reduction of ketosteroids to hydroxysteroids. Acts as a NAD(P)(H)-dependent 3-, 17- and 20-ketosteroid reductase on the steroid nucleus and side chain and regulates the metabolism of androgens, estrogens and progesterone. Displays the ability to catalyze both oxidation and reduction in vitro, but most probably acts as a reductase in vivo since the oxidase activity measured in vitro is inhibited by physiological concentration of NADPH. Acts preferentially as a 17-ketosteroid reductase and has the highest catalytic efficiency of the AKR1C enzyme for the reduction of delta4-androstenedione to form testosterone. Reduces prostaglandin (PG) D2 to 11beta-prostaglandin F2, progesterone to 20alpha-hydroxyprogesterone and estrone to 17beta-estradiol. Catalyzes the transformation of the potent androgen dihydrotestosterone (DHT) into the less active form, 5-alpha-androstan-3-alpha,17-beta-diol (3-alpha-diol). Also displays retinaldehyde reductase activity toward 9-cis-retinal. |
| Protein Name | Aldo-Keto Reductase Family 1 Member C3 17-Beta-Hydroxysteroid Dehydrogenase Type 5 17-Beta-Hsd 5 3-Alpha-Hsd Type Ii - Brain 3-Alpha-Hydroxysteroid Dehydrogenase Type 2 3-Alpha-Hsd Type 2 Chlordecone Reductase Homolog Hakrb Dihydrodiol Dehydrogenase 3 Dd-3 Dd3 Dihydrodiol Dehydrogenase Type I Ha1753 Prostaglandin F Synthase Pgfs Testosterone 17-Beta-Dehydrogenase 5 |
| Database Links | Reactome: R-HSA-193368 Reactome: R-HSA-193775 Reactome: R-HSA-193807 Reactome: R-HSA-2162123 Reactome: R-HSA-5365859 Reactome: R-HSA-975634 |
| Cellular Localisation | Cytoplasm |
| Alternative Antibody Names | Anti-Aldo-Keto Reductase Family 1 Member C3 antibody Anti-17-Beta-Hydroxysteroid Dehydrogenase Type 5 antibody Anti-17-Beta-Hsd 5 antibody Anti-3-Alpha-Hsd Type Ii - Brain antibody Anti-3-Alpha-Hydroxysteroid Dehydrogenase Type 2 antibody Anti-3-Alpha-Hsd Type 2 antibody Anti-Chlordecone Reductase Homolog Hakrb antibody Anti-Dihydrodiol Dehydrogenase 3 antibody Anti-Dd-3 antibody Anti-Dd3 antibody Anti-Dihydrodiol Dehydrogenase Type I antibody Anti-Ha1753 antibody Anti-Prostaglandin F Synthase antibody Anti-Pgfs antibody Anti-Testosterone 17-Beta-Dehydrogenase 5 antibody Anti-AKR1C3 antibody Anti-DDH1 antibody Anti-HSD17B5 antibody Anti-KIAA0119 antibody Anti-PGFS antibody |
Information sourced from Uniprot.org