• Western blot analysis of lysates from Mouse fat, using Adiponectin Rabbit monoclonal antibody (STJ11105123) at 1:2000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-ADIPOQ antibody [S5123RM] (STJ11105123)

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STJ11105123

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-Adiponectin is suitable for use in Western Blot research applications.
Clonality: Monoclonal
Clone ID: S5123RM
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:1000-1:5000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ADIPOQ
Gene ID: 9370
Uniprot ID: ADIPO_HUMAN
Immunogen: Recombinant protein of human Adiponectin.
Tissue Specificity Synthesized exclusively by adipocytes and secreted into plasma.
Post Translational Modifications HMW complexes are more extensively glycosylated than smaller oligomers. Hydroxylation and glycosylation of the lysine residues within the collagen-like domain of adiponectin seem to be critically involved in regulating the formation and/or secretion of HMW complexes and consequently contribute to the insulin-sensitizing activity of adiponectin in hepatocytes. O-glycosylated. Not N-glycosylated. O-linked glycans on hydroxylysines consist of Glc-Gal disaccharides bound to the oxygen atom of post-translationally added hydroxyl groups. Sialylated to varying degrees depending on tissue. Thr-22 appears to be the major site of sialylation. Higher sialylation found in SGBS adipocytes than in HEK fibroblasts. Sialylation is not required neither for heterodimerization nor for secretion. Not sialylated on the glycosylated hydroxylysines. Desialylated forms are rapidly cleared from the circulation. Succination of Cys-36 by the Krebs cycle intermediate fumarate, which leads to S-(2-succinyl)cysteine residues, inhibits polymerization and secretion of adiponectin. Adiponectin is a major target for succination in both adipocytes and adipose tissue of diabetic mammals. It was proposed that succination of proteins is a biomarker of mitochondrial stress and accumulation of Krebs cycle intermediates in adipose tissue in diabetes and that succination of adiponectin may contribute to the decrease in plasma adiponectin in diabetes.
Function Important adipokine involved in the control of fat metabolism and insulin sensitivity, with direct anti-diabetic, anti-atherogenic and anti-inflammatory activities. Stimulates AMPK phosphorylation and activation in the liver and the skeletal muscle, enhancing glucose utilization and fatty-acid combustion. Antagonizes TNF-alpha by negatively regulating its expression in various tissues such as liver and macrophages, and also by counteracting its effects. Inhibits endothelial NF-kappa-B signaling through a cAMP-dependent pathway. May play a role in cell growth, angiogenesis and tissue remodeling by binding and sequestering various growth factors with distinct binding affinities, depending on the type of complex, LMW, MMW or HMW.
Protein Name Adiponectin
30 Kda Adipocyte Complement-Related Protein
Adipocyte Complement-Related 30 Kda Protein
Acrp30
Adipocyte - C1q And Collagen Domain-Containing Protein
Adipose Most Abundant Gene Transcript 1 Protein
Apm-1
Gelatin-Binding Protein
Database Links Reactome: R-HSA-163680
Reactome: R-HSA-381340
Cellular Localisation Secreted
Alternative Antibody Names Anti-Adiponectin antibody
Anti-30 Kda Adipocyte Complement-Related Protein antibody
Anti-Adipocyte Complement-Related 30 Kda Protein antibody
Anti-Acrp30 antibody
Anti-Adipocyte - C1q And Collagen Domain-Containing Protein antibody
Anti-Adipose Most Abundant Gene Transcript 1 Protein antibody
Anti-Apm-1 antibody
Anti-Gelatin-Binding Protein antibody
Anti-ADIPOQ antibody
Anti-ACDC antibody
Anti-ACRP30 antibody
Anti-APM1 antibody
Anti-GBP28 antibody

Information sourced from Uniprot.org

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