• Western blot analysis of extracts of various cell lines, using ACTN2 rabbit monoclonal antibody (STJ11102400) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
  • Confocal imaging of paraffin-embedded Mouse heart using ACTN2 Rabbit monoclonal antibody (STJ11102400, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x. Perform high pressure antigen retrieval with 0. 01 M citRate buffer (pH 6. 0) prior to IF staining.
  • Immunofluorescence analysis of rat heart using ACTN2 rabbit monoclonal antibody (STJ11102400) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Confocal imaging of paraffin-embedded Rat heart using ACTN2 Rabbit monoclonal antibody (STJ11102400, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x. Perform high pressure antigen retrieval with 0. 01 M citRate buffer (pH 6. 0) prior to IF staining.
  • Immunofluorescence analysis of mouse heart using ACTN2 rabbit monoclonal antibody (STJ11102400) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Western blot analysis of various lysates using ACTN2 Rabbit monoclonal antibody (STJ11102400) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-ACTN2 antibody (1-100) [S0MR] (STJ11102400)

SKU:
STJ11102400

£212.50 - £501.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-ACTN2 (1-100) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Monoclonal
Clone ID: S0MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:1000-1:5000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ACTN2
Gene ID: 88
Uniprot ID: ACTN2_HUMAN
Immunogen Region: 1-100
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 1-100 of human ACTN2 (P35609).
Immunogen Sequence: MNQIEPGVQYNYVYDEDEYM IQEEEWDRDLLLDPAWEKQQ RKTFTAWCNSHLRKAGTQIE NIEEDFRNGLKLMLLLEVIS GERLPKPDRGKMRFHKIANV
Tissue Specificity Expressed in both skeletal and cardiac muscle.
Post Translational Modifications Ubiquitinated by FBXL22, leading to proteasomal degradation.
Function F-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. This is a bundling protein.
Protein Name Alpha-Actinin-2
Alpha-Actinin Skeletal Muscle Isoform 2
F-Actin Cross-Linking Protein
Database Links Reactome: R-HSA-114608
Reactome: R-HSA-373753
Reactome: R-HSA-390522
Reactome: R-HSA-438066
Reactome: R-HSA-442982
Reactome: R-HSA-5673001
Reactome: R-HSA-9609736
Reactome: R-HSA-9617324
Reactome: R-HSA-9620244
Cellular Localisation Cytoplasm
Myofibril
Sarcomere
Z Line
Colocalizes With Myoz1 And Flnc At The Z-Lines Of Skeletal Muscle
Alternative Antibody Names Anti-Alpha-Actinin-2 antibody
Anti-Alpha-Actinin Skeletal Muscle Isoform 2 antibody
Anti-F-Actin Cross-Linking Protein antibody
Anti-ACTN2 antibody

Information sourced from Uniprot.org

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