• Western blot analysis of various lysates using ACTL9 Rabbit polyclonal antibody (STJ118419) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-ACTL9 antibody (1-416) (STJ118419)

SKU:
STJ118419

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Host: Rabbit
Applications: WB/ELISA
Reactivity: Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:2000
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ACTL9
Gene ID: 284382
Uniprot ID: ACTL9_HUMAN
Immunogen Region: 1-416
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 1-416 of human ACTL9 (NP_848620.3).
Immunogen Sequence: MDASRPKSSESQSSLEAPRP GPNPSPNVVNKPLQRDSPGM VADRLPPKTGAVVIDMGTGT CKVGFAGQASPTYTVATILG CQPKKPATSGQSGLQTFIGE AARVLPELTLVQPLRSGIVV DWDAAELIWRHLLEHDLRVA THDHPLLFSDPPFSPATNRE KLVEVAFESLRSPAMYVASQ SVLSVYAHGRVSGLVVDTGH GVTYTVPVFQGYNLLHATER LDLAGNNLTAFLAEMLLQA
Tissue Specificity Testis-specific.
Function Testis-specic protein that plays an important role in fusion of proacrosomal vesicles and perinuclear theca formation.
Protein Name Actin-Like Protein 9
Cellular Localisation Cytoplasmic Vesicle
Secretory Vesicle
Acrosome
Cytoplasm
Cytoskeleton
Perinuclear Theca
Localizes Predominantly In The Equatorial Segment Of The Sperm Head And Neck Regions
With Some Localization In The Acrosomal Segment Of The Head
Colocalizes In The Acrosomal And Equatorial Segments Of Sperm With Actl7a
Colocalizes With Plcz1 In The Equatorial Segment Of The Head Of Capacitated Sperm
Alternative Antibody Names Anti-Actin-Like Protein 9 antibody
Anti-ACTL9 antibody
Anti-ACTL7C antibody
Anti-HSD21 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance