• Human uterus stained with anti-SMA antibody using peroxidase-conjugate and DAB chromogen. Note the cytoplasmic staining of smooth muscle.

Anti-ACTA2 antibody (N-Term) [1A4] (STJ180001)


Current Stock:
Host: Mouse
Applications: IHC-P
Reactivity: Human
Short Description: Mouse monoclonal antibody anti-ACTA2 (N-Term) is suitable for use in Immunohistochemistry research applications.
Clonality: Monoclonal
Clone ID: 1A4
Conjugation: Unconjugated
Isotype: IgG2a/Kappa
Formulation: Liquid in buffer with protein carrier and preservative
Purification: Affinity purified
Dilution Range: 1:100
Storage Instruction: Store at 2‐8°C for up to 24 months. Predilute: Ready to use, no reconstitution necessary. Concentrate: Use dilution range and appropriate lab‐standardized diluent. Stability after dilution: 7 days at 24°C, 3 months at 2‐8°C, 6months at ‐20°C.
Gene Symbol: ACTA2
Gene ID: 59
Immunogen Region: N-Term
Specificity: Positive control: Smooth muscle or leiomyosarcoma
Immunogen: N-Terminal decapeptide of alpha smooth muscle isoform of actin; acetylated at the N-terminus
Post Translational Modifications Oxidation of Met-46 and Met-49 by MICALs (MICAL1, MICAL2 or MICAL3) to form methionine sulfoxide promotes actin filament depolymerization. MICAL1 and MICAL2 produce the (R)-S-oxide form. The (R)-S-oxide form is reverted by MSRB1 and MSRB2, which promotes actin repolymerization. Monomethylation at Lys-86 (K84me1) regulates actin-myosin interaction and actomyosin-dependent processes. Demethylation by ALKBH4 is required for maintaining actomyosin dynamics supporting normal cleavage furrow ingression during cytokinesis and cell migration. Methylated at His-75 by SETD3. (Microbial infection) Monomeric actin is cross-linked by V.cholerae toxins RtxA and VgrG1 in case of infection: bacterial toxins mediate the cross-link between Lys-52 of one monomer and Glu-272 of another actin monomer, resulting in formation of highly toxic actin oligomers that cause cell rounding. The toxin can be highly efficient at very low concentrations by acting on formin homology family proteins: toxic actin oligomers bind with high affinity to formins and adversely affect both nucleation and elongation abilities of formins, causing their potent inhibition in both profilin-dependent and independent manners.
Function Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
Protein Name Actin - Aortic Smooth Muscle
Cell Growth-Inhibiting Gene 46 Protein Cleaved Into - Actin - Aortic Smooth Muscle - Intermediate Form
Database Links Reactome: R-HSA-445355
Reactome: R-HSA-9013695
Cellular Localisation Cytoplasm
Alternative Antibody Names Anti-Actin - Aortic Smooth Muscle antibody
Anti-Alpha-Actin-2 antibody
Anti-Cell Growth-Inhibiting Gene 46 Protein Cleaved Into - Actin - Aortic Smooth Muscle - Intermediate Form antibody
Anti-ACTA2 antibody
Anti-ACTSA antibody
Anti-ACTVS antibody
Anti-GIG46 antibody

Information sourced from Uniprot.org

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