Anti-ACTA1 antibody (250-350) [S5MR] (STJ11101805)

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STJ11101805
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Host: Rabbit
Applications: WB/IF/ICC/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality : Monoclonal
Clone ID : S5MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:1000
IF/ICC:1:50-1:200
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ACTA1
Gene ID: 58
Uniprot ID: ACTS_HUMAN
Immunogen Region: 250-350
Specificity: A synthetic peptide corresponding to a sequence within amino acids 250-350 of human Alpha-Actin-1 (ACTA1) (P68133).
Immunogen Sequence: ITIGNERFRCPETLFQPSFI GMESAGIHETTYNSIMKCDI DIRKDLYANNVMSGGTTMYP GIADRMQKEITALAPSTMKI KIIAPPERKYSVWIGGSILA S
Post Translational Modifications Oxidation of Met-46 and Met-49 by MICALs (MICAL1, MICAL2 or MICAL3) to form methionine sulfoxide promotes actin filament depolymerization. MICAL1 and MICAL2 produce the (R)-S-oxide form. The (R)-S-oxide form is reverted by MSRB1 and MSRB2, which promotes actin repolymerization. Monomethylation at Lys-86 (K84me1) regulates actin-myosin interaction and actomyosin-dependent processes. Demethylation by ALKBH4 is required for maintaining actomyosin dynamics supporting normal cleavage furrow ingression during cytokinesis and cell migration. Actin, alpha skeletal muscle, intermediate form: N-terminal cleavage of acetylated cysteine of intermediate muscle actin by ACTMAP. Methylated at His-75 by SETD3. (Microbial infection) Monomeric actin is cross-linked by V.cholerae toxins RtxA and VgrG1 in case of infection: bacterial toxins mediate the cross-link between Lys-52 of one monomer and Glu-272 of another actin monomer, resulting in formation of highly toxic actin oligomers that cause cell rounding. The toxin can be highly efficient at very low concentrations by acting on formin homology family proteins: toxic actin oligomers bind with high affinity to formins and adversely affect both nucleation and elongation abilities of formins, causing their potent inhibition in both profilin-dependent and independent manners.
Function Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
Protein Name Actin - Alpha Skeletal Muscle
Alpha-Actin-1 Cleaved Into - Actin - Alpha Skeletal Muscle - Intermediate Form
Database Links Reactome: R-HSA-390522
Cellular Localisation Cytoplasm
Cytoskeleton
Alternative Antibody Names Anti-Actin - Alpha Skeletal Muscle antibody
Anti-Alpha-Actin-1 Cleaved Into - Actin - Alpha Skeletal Muscle - Intermediate Form antibody
Anti-ACTA1 antibody
Anti-ACTA antibody

Information sourced from Uniprot.org