• Western blot analysis of various lysates using ACSF3 Rabbit polyclonal antibody (STJ115715) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-ACSF3 antibody (270-450) (STJ115715)

SKU:
STJ115715

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-ACSF3 (270-450) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ACSF3
Gene ID: 197322
Uniprot ID: ACSF3_HUMAN
Immunogen Region: 270-450
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 270-450 of human ACSF3 (NP_001230208.1).
Immunogen Sequence: FSPQQVWEKFLSSETPRINV FMAVPTIYTKLMEYYDRHFT QPHAQDFLRAVCEEKIRLMV SGSAALPLPVLEKWKNITGH TLLERYGMTEIGMALSGPLT TAVRLPGSVGTPLPGVQVRI VSENPQREACSYTIHAEGDE RGTKVTPGFEEKEGELLVRG PSVFREYWNKPEETKSAFTL D
Function Catalyzes the initial reaction in intramitochondrial fatty acid synthesis, by activating malonate and methylmalonate, but not acetate, into their respective CoA thioester. May have some preference toward very-long-chain substrates.
Protein Name Malonate--Coa Ligase Acsf3 - Mitochondrial
Acyl-Coa Synthetase Family Member 3
Database Links Reactome: R-HSA-75876
Cellular Localisation Mitochondrion
Alternative Antibody Names Anti-Malonate--Coa Ligase Acsf3 - Mitochondrial antibody
Anti-Acyl-Coa Synthetase Family Member 3 antibody
Anti-ACSF3 antibody
Anti-PSEC0197 antibody

Information sourced from Uniprot.org

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