• Immunofluorescence analysis of NIH-3T3 cells using PABPN1 Rabbit mAb (STJ11101437) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of U-2 OS cells using PABPN1 Rabbit mAb (STJ11101437) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of C6 cells using PABPN1 Rabbit mAb (STJ11101437) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunohistochemistry of paraffin-embedded mouse testis using PABPN1 Rabbit mAb (STJ11101437) at dilution of 1:100 (40x lens).
  • Immunohistochemistry of paraffin-embedded human esophageal using PABPN1 Rabbit mAb (STJ11101437) at dilution of 1:100 (40x lens).
  • Immunohistochemistry of paraffin-embedded rat brain using PABPN1 Rabbit mAb (STJ11101437) at dilution of 1:100 (40x lens).
  • Western blot analysis of extracts of various cell lines, using PABPN1 Rabbit mAb (STJ11101437) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 10s.

Anti-Acetyl-p53-K382 antibody (STJ11101437)

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STJ11101437
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Host: Rabbit
Applications: WB
Reactivity: Human, Mouse
Short Description: Rabbit monoclonal primary antibody against TP53.
Note: FOR RESEARCH USE ONLY (RUO).
Clonality: Monoclonal
Conjugation: Unconjugated
Formulation: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Purification: Affinity purification
Storage Instruction: Store at-20°C. Avoid freeze/thaw cycles.
Isotype: IgG
Dilution Range: WB: 1:500-1:2000
Uniprot ID: P53_HUMAN
Gene ID: 7157
Gene Symbol: TP53
Immunogen: A synthetic acetylated peptide around K382 of human p53 (NP_000537.3).
Tissue Specificity Ubiquitous. Isoforms are expressed in a wide range of normal tissues but in a tissue-dependent manner. Isoform 2 is expressed in most normal tissues but is not detected in brain, lung, prostate, muscle, fetal brain, spinal cord and fetal liver. Isoform 3 is expressed in most normal tissues but is not detected in lung, spleen, testis, fetal brain, spinal cord and fetal liver. Isoform 7 is expressed in most normal tissues but is not detected in prostate, uterus, skeletal muscle and breast. Isoform 8 is detected only in colon, bone marrow, testis, fetal brain and intestine. Isoform 9 is expressed in most normal tissues but is not detected in brain, heart, lung, fetal liver, salivary gland, breast or intestine.
Database Links Reactome: R-HSA-111448
Cellular Localisation Cytoplasm
Nucleus
Pml Body
Endoplasmic Reticulum
Mitochondrion Matrix
Cytoskeleton
Microtubule Organizing Center
Centrosome
Interaction With Banp Promotes Nuclear Localization
Recruited Into Pml Bodies Together With Chek2
Translocates To Mitochondria Upon Oxidative Stress
Translocates To Mitochondria In Response To Mitomycin C Treatment
Isoform 1: Nucleus
Predominantly Nuclear But Localizes To The Cytoplasm When Expressed With Isoform 4
Isoform 2: Nucleus
Localized Mainly In The Nucleus With Minor Staining In The Cytoplasm
Isoform 3: Nucleus
Localized In The Nucleus In Most Cells But Found In The Cytoplasm In Some Cells
Isoform 4: Nucleus
Predominantly Nuclear But Translocates To The Cytoplasm Following Cell Stress
Isoform 7: Nucleus
Isoform 8: Nucleus
Localized In Both Nucleus And Cytoplasm In Most Cells
In Some Cells
Forms Foci In The Nucleus That Are Different From Nucleoli
Isoform 9: Cytoplasm
Protein Name Cellular Tumor Antigen P53
Antigen Ny-Co-13
Phosphoprotein P53
Tumor Suppressor P53
Alternative Names Anti-Cellular Tumor Antigen P53 antibody
Anti-Antigen Ny-Co-13 antibody
Anti-Phosphoprotein P53 antibody
Anti-Tumor Suppressor P53 antibody
Anti-TP53 antibody
Anti-P53 antibody

Information sourced from uniprot.org

6 months standard