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Anti-Acetyl-Histone H4-Lys16 antibody (1-50 aa) (STJ90129)

Immunofluorescence analysis of Human-lung-cancer tissue. 1, Histone H4 (Acetyl Lys16) Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
Immunofluorescence analysis of Human-lung tissue. 1, Histone H4 (Acetyl Lys16) Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
Immunofluorescence analysis of Human-lung-cancer tissue. 1, Histone H4 (Acetyl Lys16) Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
Immunofluorescence analysis of Human-lung-cancer tissue. 1, Histone H4 (Acetyl Lys16) Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
Immunofluorescence analysis of HeLa cells, using Histone H4 (Acetyl-Lys16) Antibody. The picture on the right is blocked with the synthesized peptide.
Immunohistochemical analysis of paraffin-embedded Human-breast tissue. 1, Histone H4 (Acetyl Lys16) Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
Immunohistochemical analysis of paraffin-embedded Human-stomach-cancer tissue. 1, Histone H4 (Acetyl Lys16) Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
Western blot analysis of various cells using Acetyl-Histone H4 (K16) Polyclonal Antibody. Secondary antibody was diluted at 1:20000
Immunohistochemical analysis of paraffin-embedded Human-lung-cancer tissue. 1, Histone H4 (Acetyl Lys16) Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
Immunohistochemical analysis of paraffin-embedded Human-kidney-cancer tissue. 1, Histone H4 (Acetyl Lys16) Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
Immunohistochemical analysis of paraffin-embedded Human-lung tissue. 1, Histone H4 (Acetyl Lys16) Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
Immunohistochemical analysis of paraffin-embedded Human-liver-cancer tissue. 1, Histone H4 (Acetyl Lys16) Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
Immunohistochemical analysis of paraffin-embedded Human-breast-cancer tissue. 1, Histone H4 (Acetyl Lys16) Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
Immunofluorescence analysis of Human-lung tissue. 1, Histone H4 (Acetyl Lys16) Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
Immunofluorescence analysis of Human-kidney tissue. 1, Histone H4 (Acetyl Lys16) Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
Immunofluorescence analysis of Human-lung tissue. 1, Histone H4 (Acetyl Lys16) Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
Immunofluorescence analysis of Human-kidney tissue. 1, Histone H4 (Acetyl Lys16) Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
Western blot analysis of KB cells using Acetyl-Histone H4 (K16) Polyclonal Antibody. Secondary antibody was diluted at 1:20000
Immunofluorescence analysis of Human-kidney tissue. 1, Histone H4 (Acetyl Lys16) Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using Histone H4 (Acetyl-Lys16) Antibody. The picture on the right is blocked with the synthesized peptide.
Immunofluorescence analysis of Hela cell. 1, Histone H4 (Acetyl Lys16) Polyclonal Antibody (red) was diluted at 1:200 (4°C overnight). Alpha-tubulin monoclonal antibody (8F11) (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 594 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min).

Anti-Acetyl-Histone H4-Lys16 antibody (1-50 aa) (STJ90129)

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STJ90129

€57.50 - €282.50

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General Information
Product Properties
Target Information
Additional Info
Warranty Information

Host:	Rabbit
Applications:	WB/IHC/IF/ELISA
Reactivity:	Human/Mouse/Rat
Note:	STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description:	Rabbit polyclonal antibody anti-Acetyl-Histone H4-Lys16 (1-50 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications.

Clonality:	Polyclonal
Conjugation:	Unconjugated
Isotype:	IgG
Formulation:	Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification:	The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration:	1 mg/mL
Dilution Range:	WB 1:500-2000 IHC-P 1:50-300 IF 1:50-300
Storage Instruction:	Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.

Gene Symbol:	H4C1.H4C2.H4C3.H4C4.H4C5.H4C6.H4C8.H4C9.H4C11.H4C12.H4C13.H4C14.H4C15.H4C16
Gene ID:	121504/554313/8294/8359/8360/8361/8362/8363/8364/8365/8366/8367/8368/8370
Uniprot ID:	H4_HUMAN
Immunogen Region:	1-50 aa
Specificity:	Acetyl-Histone H4 (K16) Polyclonal Antibody detects endogenous levels of Histone H4 protein only when acetylated at K16.
Immunogen:	The antiserum was produced against synthesized peptide derived from the human Histone H4 around the acetylated site of Lys16 at the amino acid range 1-50

Function	Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
Protein Name	Histone H4
Database Links	Reactome: R-HSA-110328 Reactome: R-HSA-110329 Reactome: R-HSA-110330 Reactome: R-HSA-110331 Reactome: R-HSA-1221632 Reactome: R-HSA-171306 Reactome: R-HSA-1912408 Reactome: R-HSA-201722 Reactome: R-HSA-212300 Reactome: R-HSA-2299718 Reactome: R-HSA-2559580 Reactome: R-HSA-2559582 Reactome: R-HSA-2559586 Reactome: R-HSA-3214815 Reactome: R-HSA-3214841 Reactome: R-HSA-3214842 Reactome: R-HSA-3214847 Reactome: R-HSA-3214858 Reactome: R-HSA-427359 Reactome: R-HSA-427389 Reactome: R-HSA-427413 Reactome: R-HSA-4551638 Reactome: R-HSA-5250924 Reactome: R-HSA-5334118 Reactome: R-HSA-5578749 Reactome: R-HSA-5617472 Reactome: R-HSA-5625886 Reactome: R-HSA-5693565 Reactome: R-HSA-5693571 Reactome: R-HSA-5693607 Reactome: R-HSA-606279 Reactome: R-HSA-68616 Reactome: R-HSA-69473 Reactome: R-HSA-73728 Reactome: R-HSA-73772 Reactome: R-HSA-8936459 Reactome: R-HSA-8939236 Reactome: R-HSA-9018519 Reactome: R-HSA-912446 Reactome: R-HSA-9609690 Reactome: R-HSA-9610379 Reactome: R-HSA-9616222 Reactome: R-HSA-9670095 Reactome: R-HSA-9710421 Reactome: R-HSA-977225
Cellular Localisation	Nucleus Chromosome
Alternative Antibody Names	Anti-Histone H4 antibody Anti-H4C1 antibody Anti-H4 antibody Anti-A antibody Anti-H4FA antibody Anti-HIST1H4A.H4C2 antibody Anti-H4 antibody Anti-I antibody Anti-H4FI antibody Anti-HIST1H4B.H4C3 antibody Anti-H4 antibody Anti-G antibody Anti-H4FG antibody Anti-HIST1H4C.H4C4 antibody Anti-H4 antibody Anti-B antibody Anti-H4FB antibody Anti-HIST1H4D.H4C5 antibody Anti-H4 antibody Anti-J antibody Anti-H4FJ antibody Anti-HIST1H4E.H4C6 antibody Anti-H4 antibody Anti-C antibody Anti-H4FC antibody Anti-HIST1H4F.H4C8 antibody Anti-H4 antibody Anti-H antibody Anti-H4FH antibody Anti-HIST1H4H.H4C9 antibody Anti-H4 antibody Anti-M antibody Anti-H4FM antibody Anti-HIST1H4I.H4C11 antibody Anti-H4 antibody Anti-E antibody Anti-H4FE antibody Anti-HIST1H4J.H4C12 antibody Anti-H4 antibody Anti-D antibody Anti-H4FD antibody Anti-HIST1H4K.H4C13 antibody Anti-H4 antibody Anti-K antibody Anti-H4FK antibody Anti-HIST1H4L.H4C14 antibody Anti-H4 antibody Anti-N antibody Anti-H4F2 antibody Anti-H4FN antibody Anti-HIST2H4 antibody Anti-HIST2H4A.H4C15 antibody Anti-H4 antibody Anti-O antibody Anti-H4FO antibody Anti-HIST2H4B.H4C16 antibody Anti-H4-16 antibody Anti-HIST4H4 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance

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