• Chromatin immunoprecipitation analysis of extracts of HeLa cells, using Acetyl-Histone H4-K5 antibody (STJ117427) and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.
  • Immunohistochemistry of paraffin-embedded mouse brain using H4K5AC antibody (STJ117427) at dilution of 1:100 (40x lens).
  • Immunohistochemistry of paraffin-embedded human stomach using H4K5AC antibody (STJ117427) at dilution of 1:100 (40x lens).
  • Immunohistochemistry of paraffin-embedded human tonsil using H4K5AC antibody (STJ117427) at dilution of 1:100 (40x lens).
  • Immunohistochemistry of paraffin-embedded rat brain using H4K5AC antibody (STJ117427) at dilution of 1:100 (40x lens).
  • Western blot analysis of extracts of various cell lines, using Acetyl-Histone H4-K5 antibody (STJ117427) at 1:1000 dilution. HeLa cells were treated by TSA (1uM) for 18 hours. C6 cells were treated by TSA (1uM) for 18 hours.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.

Anti-Acetyl-Histone H4-K5 antibody (STJ117427)


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Host: Rabbit
Applications: WB, IHC, IF, ChIP
Reactivity: Human, Mouse, Rat, Other
Short Description: Rabbit polyclonal anti-Acetyl-Histone H4-K5 antibody is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ChImmunoprecipitation.
Clonality: Polyclonal
Conjugation: Unconjugated
Formulation: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Purification: Affinity purification
Storage Instruction: Store at-20°C. Avoid freeze/thaw cycles.
Isotype: IgG
Dilution Range: WB: 1:500-1:2000
IHC: 1:50-1:200
IF: 1:50-1:200
ChIP: 1:20-1:100
Uniprot ID: H4_HUMAN
Gene ID: 121504/554313/8294/8359/8360/8361/8362/8363/8364/8365/8366/8367/8368/8370
Gene Symbol: H4C1.H4C2.H4C3.H4C4.H4C5.H4C6.H4C8.H4C9.H4C11.H4C12.H4C13.H4C14.H4C15.H4-16
Immunogen: A synthetic acetylated peptide around K5 of human Histone H4 (NP_001029249.1).
Post Translational Modifications Acetylation at Lys-6 (H4K5ac), Lys-9 (H4K8ac), Lys-13 (H4K12ac) and Lys-17 (H4K16ac) occurs in coding regions of the genome but not in heterochromatin. Citrullination at Arg-4 (H4R3ci) by PADI4 impairs methylation. Monomethylation and asymmetric dimethylation at Arg-4 (H4R3me1 and H4R3me2a, respectively) by PRMT1 favors acetylation at Lys-9 (H4K8ac) and Lys-13 (H4K12ac). Demethylation is performed by JMJD6. Symmetric dimethylation on Arg-4 (H4R3me2s) by the PRDM1/PRMT5 complex may play a crucial role in the germ-cell lineage. Monomethylated, dimethylated or trimethylated at Lys-21 (H4K20me1, H4K20me2, H4K20me3). Monomethylation is performed by KMT5A/SET8. Dimethylation and trimethylation is performed by KMT5B and KMT5C and induces gene silencing. Monomethylated at Lys-13 (H4K12me1) by N6AMT1.H4K12me1 modification is present at the promoters of numerous genes encoding cell cycle regulators. Phosphorylated by PAK2 at Ser-48 (H4S47ph). This phosphorylation increases the association of H3.3-H4 with the histone chaperone HIRA, thus promoting nucleosome assembly of H3.3-H4 and inhibiting nucleosome assembly of H3.1-H4. Ubiquitinated by the CUL4-DDB-RBX1 complex in response to ultraviolet irradiation. This may weaken the interaction between histones and DNA and facilitate DNA accessibility to repair proteins. Monoubiquitinated at Lys-92 of histone H4 (H4K91ub1) in response to DNA damage. The exact role of H4K91ub1 in DNA damage response is still unclear but it may function as a licensing signal for additional histone H4 post-translational modifications such as H4 Lys-21 methylation (H4K20me). Ufmylated.monofmylated by UFL1 at Lys-32 (H4K31Ufm1) in response to DNA damage. Sumoylated, which is associated with transcriptional repression. Crotonylation (Kcr) is specifically present in male germ cells and marks testis-specific genes in post-meiotic cells, including X-linked genes that escape sex chromosome inactivation in haploid cells. Crotonylation marks active promoters and enhancers and confers resistance to transcriptional repressors. It is also associated with post-meiotically activated genes on autosomes. Butyrylation of histones marks active promoters and competes with histone acetylation. Glutarylation at Lys-92 (H4K91glu) destabilizes nucleosomes by promoting dissociation of the H2A-H2B dimers from nucleosomes.
Function Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
Protein Name Histone H4
Database Links Reactome: R-HSA-110328
Reactome: R-HSA-110329
Reactome: R-HSA-110330
Reactome: R-HSA-110331
Reactome: R-HSA-1221632
Reactome: R-HSA-171306
Reactome: R-HSA-1912408
Reactome: R-HSA-201722
Reactome: R-HSA-212300
Reactome: R-HSA-2299718
Reactome: R-HSA-2559580
Reactome: R-HSA-2559582
Reactome: R-HSA-2559586
Reactome: R-HSA-3214815
Reactome: R-HSA-3214841
Reactome: R-HSA-3214842
Reactome: R-HSA-3214847
Reactome: R-HSA-3214858
Reactome: R-HSA-427359
Reactome: R-HSA-427389
Reactome: R-HSA-427413
Reactome: R-HSA-4551638
Reactome: R-HSA-5250924
Reactome: R-HSA-5334118
Reactome: R-HSA-5578749
Reactome: R-HSA-5617472
Reactome: R-HSA-5625886
Reactome: R-HSA-5693565
Reactome: R-HSA-5693571
Reactome: R-HSA-5693607
Reactome: R-HSA-606279
Reactome: R-HSA-69473
Reactome: R-HSA-73728
Reactome: R-HSA-73772
Reactome: R-HSA-8936459
Reactome: R-HSA-8939236
Reactome: R-HSA-9018519
Reactome: R-HSA-912446
Reactome: R-HSA-9609690
Reactome: R-HSA-9610379
Reactome: R-HSA-9616222
Reactome: R-HSA-9670095
Reactome: R-HSA-977225
Cellular Localisation Nucleus
Alternative Antibody Names Anti-Histone H4 antibody
Anti-H4C1 antibody
Anti-H4 antibody
Anti-A antibody
Anti-H4FA antibody
Anti-HIST1H4A.H4C2 antibody
Anti-H4 antibody
Anti-I antibody
Anti-H4FI antibody
Anti-HIST1H4B.H4C3 antibody
Anti-H4 antibody
Anti-G antibody
Anti-H4FG antibody
Anti-HIST1H4C.H4C4 antibody
Anti-H4 antibody
Anti-B antibody
Anti-H4FB antibody
Anti-HIST1H4D.H4C5 antibody
Anti-H4 antibody
Anti-J antibody
Anti-H4FJ antibody
Anti-HIST1H4E.H4C6 antibody
Anti-H4 antibody
Anti-C antibody
Anti-H4FC antibody
Anti-HIST1H4F.H4C8 antibody
Anti-H4 antibody
Anti-H antibody
Anti-H4FH antibody
Anti-HIST1H4H.H4C9 antibody
Anti-H4 antibody
Anti-M antibody
Anti-H4FM antibody
Anti-HIST1H4I.H4C11 antibody
Anti-H4 antibody
Anti-E antibody
Anti-H4FE antibody
Anti-HIST1H4J.H4C12 antibody
Anti-H4 antibody
Anti-D antibody
Anti-H4FD antibody
Anti-HIST1H4K.H4C13 antibody
Anti-H4 antibody
Anti-K antibody
Anti-H4FK antibody
Anti-HIST1H4L.H4C14 antibody
Anti-H4 antibody
Anti-N antibody
Anti-H4F2 antibody
Anti-H4FN antibody
Anti-HIST2H4 antibody
Anti-HIST2H4A.H4C15 antibody
Anti-H4 antibody
Anti-O antibody
Anti-H4FO antibody
Anti-HIST2H4B.H4-16 antibody
Anti-HIST4H4 antibody

Information sourced from Uniprot.org

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