• Western blot analysis of 3T3 mouse-kidney KB K562 Hela lysate, antibody was diluted at 500. Secondary antibody was diluted at 1:20000

Anti-Acetyl-FOXO4-Lys189 antibody (189) (STJ98685)


Current Stock:
Host: Rabbit
Applications: WB, ELISA
Reactivity: Human, Rat, Mouse
Short Description: Rabbit polyclonal antibody anti-Acetyl-Forkhead Box Protein O4-Lys189 (189) is suitable for use in Western Blot and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS, 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit anti-serum by affinity-chromatography.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-2000
ELISA 1:10000-20000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: FOXO4
Gene ID: 4303
Immunogen Region: 189
Specificity: Acetyl-FOXO4-Lys189 polyclonal antibody (Forkhead Box Protein O4) binds to endogenous Forkhead Box Protein O4 at the amino acid region 189.
Immunogen: Synthetic Acetyl peptide from human protein at amino acid range: 189
Post Translational Modifications Acetylation by CREBBP/CBP, which is induced by peroxidase stress, inhibits transcriptional activity. Deacetylation by SIRT1 is NAD-dependent and stimulates transcriptional activity. Phosphorylation by PKB/AKT1 inhibits transcriptional activity and is responsible for cytoplasmic localization. May be phosphorylated at multiple sites by NLK. Monoubiquitinated.monoubiquitination is induced by oxidative stress and reduced by deacetylase inhibitors.results in its relocalization to the nucleus and its increased transcriptional activity. Deubiquitinated by USP7.deubiquitination is induced by oxidative stress.enhances its interaction with USP7 and consequently, deubiquitination.increases its translocation to the cytoplasm and inhibits its transcriptional activity. Hydrogene-peroxide-induced ubiquitination and USP7-mediated deubiquitination have no major effect on its protein stability.
Function Transcription factor involved in the regulation of the insulin signaling pathway. Binds to insulin-response elements (IREs) and can activate transcription of IGFBP1. Down-regulates expression of HIF1A and suppresses hypoxia-induced transcriptional activation of HIF1A-modulated genes. Also involved in negative regulation of the cell cycle. Involved in increased proteasome activity in embryonic stem cells (ESCs) by activating expression of PSMD11 in ESCs, leading to enhanced assembly of the 26S proteasome, followed by higher proteasome activity.
Protein Name Forkhead Box Protein O4
Fork Head Domain Transcription Factor Afx1
Database Links Reactome: R-HSA-198693
Reactome: R-HSA-5674400
Reactome: R-HSA-5689880
Reactome: R-HSA-9614399
Reactome: R-HSA-9614657
Reactome: R-HSA-9615017
Reactome: R-HSA-9617629
Reactome: R-HSA-9617828
Cellular Localisation Cytoplasm
When Phosphorylated
Translocated From Nucleus To Cytoplasm
Dephosphorylation Triggers Nuclear Translocation
Monoubiquitination Increases Nuclear Localization
When Deubiquitinated
Alternative Antibody Names Anti-Forkhead Box Protein O4 antibody
Anti-Fork Head Domain Transcription Factor Afx1 antibody
Anti-FOXO4 antibody
Anti-AFX antibody
Anti-AFX1 antibody
Anti-MLLT7 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance