• Immunofluorescence analysis of 293T cells using AARS Rabbit polyclonal antibody (STJ117211) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of AARS in paraffin-embedded mouse liver using AARS Rabbit polyclonal antibody (STJ117211) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of AARS in paraffin-embedded human lung cancer using AARS Rabbit polyclonal antibody (STJ117211) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of AARS in paraffin-embedded rat spleen using AARS Rabbit polyclonal antibody (STJ117211) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates using AARS Rabbit polyclonal antibody (STJ117211) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-AARS antibody (1-100) (STJ117211)

SKU:
STJ117211

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-AARS (1-100) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: AARS1
Gene ID: 16
Uniprot ID: SYAC_HUMAN
Immunogen Region: 1-100
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 1-100 of human AARS (NP_001596.2).
Immunogen Sequence: MDSTLTASEIRQRFIDFFKR NEHTYVHSSATIPLDDPTLL FANAGMNQFKPIFLNTIDPS HPMAKLSRAANTQKCIRAGG KHNDLDDVGKDVYHHTFFEM
Post Translational Modifications ISGylated.
Function Catalyzes the attachment of alanine to tRNA(Ala) in a two-step reaction: alanine is first activated by ATP to form Ala-AMP and then transferred to the acceptor end of tRNA(Ala). Also edits incorrectly charged tRNA(Ala) via its editing domain.
Protein Name Alanine--Trna Ligase - Cytoplasmic
Alanyl-Trna Synthetase
Alars
Renal Carcinoma Antigen Ny-Ren-42
Database Links Reactome: R-HSA-379716
Cellular Localisation Cytoplasm
Alternative Antibody Names Anti-Alanine--Trna Ligase - Cytoplasmic antibody
Anti-Alanyl-Trna Synthetase antibody
Anti-Alars antibody
Anti-Renal Carcinoma Antigen Ny-Ren-42 antibody
Anti-AARS1 antibody
Anti-AARS antibody

Information sourced from Uniprot.org

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