• Western blot analysis of extracts of various cell lines, using AAAS antibody (STJ28510) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit . Exposure time: 30s.
  • Immunofluorescence analysis of HeLa cells using AAAS antibody (STJ28510). Blue: DAPI for nuclear staining.

Anti-AAAS-antibody (aa287-546) (STJ28510)

SKU:
STJ28510

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Host: Rabbit
Applications: WB, IF
Reactivity: Human
Note: FOR RESEARCH USE ONLY (RUO).
Short Description: Rabbit polyclonal antibody anti-AAAS is suitable for use in Western Blot and Immunofluorescence.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF 1:50-1:200
Storage Instruction: Store at-20°C. Avoid freeze/thaw cycles.
Gene Symbol: AAAS
Gene ID: 8086
Uniprot ID: AAAS_HUMAN
Immunogen Region: 287-546
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 287-546 of human AAAS (NP_056480.1).
Tissue Specificity Widely expressed. Particularly abundant in cerebellum, corpus callosum, adrenal gland, pituitary gland, gastrointestinal structures and fetal lung.
Function Plays a role in the normal development of the peripheral and central nervous system. Required for the correct localization of aurora kinase AURKA and the microtubule minus end-binding protein NUMA1 as well as a subset of AURKA targets which ensures proper spindle formation and timely chromosome alignment.
Protein Name Aladin
Adracalin
Database Links Reactome: R-HSA-1169408
Reactome: R-HSA-159227
Reactome: R-HSA-159230
Reactome: R-HSA-159231
Reactome: R-HSA-159236
Reactome: R-HSA-165054
Reactome: R-HSA-168271
Reactome: R-HSA-168276
Reactome: R-HSA-168325
Reactome: R-HSA-168333
Reactome: R-HSA-170822
Reactome: R-HSA-180746
Reactome: R-HSA-180910
Reactome: R-HSA-191859
Reactome: R-HSA-3108214
Reactome: R-HSA-3232142
Reactome: R-HSA-3301854
Reactome: R-HSA-3371453
Reactome: R-HSA-4085377
Reactome: R-HSA-4551638
Reactome: R-HSA-4570464
Reactome: R-HSA-4615885
Reactome: R-HSA-5578749
Reactome: R-HSA-5619107
Reactome: R-HSA-6784531
Reactome: R-HSA-9609690
Reactome: R-HSA-9610379
Cellular Localisation Nucleus
Nuclear Pore Complex
Cytoplasm
Cytoskeleton
Spindle Pole
Nucleus Envelope
In Metaphase Cells Localizes Within The Spindle With Some Accumulation Around Spindle Poles
With The Highest Concentration Between The Centrosome And Metaphase Plate
The Localization To The Spindle Is Microtubule-Mediated
Alternative Antibody Names Anti-Aladin antibody
Anti-Adracalin antibody
Anti-AAAS antibody
Anti-ADRACALA antibody
Anti-GL003 antibody

Information sourced from Uniprot.org

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