| Applications: |
Immunodepletion/Immunocompetition |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
SPHK1 Blocking Peptide for STJ503118 is synthetically produced from the 50-100 sequence and is suitable for use in western blot applications. |
| Formulation: |
Liquid form at 2.5mg/ml concentration in PBS. Up to 5% DMSO can be added. Orders with >1mg can be supplied in lyophilized powder form, or in buffer of choice. |
| Storage Instruction: |
Store at-20°C for long term storage. Avoid freeze-thaw cycles. |
| Gene Symbol: |
Sphk1 |
| Gene ID: |
20698 |
| Uniprot ID: |
SPHK1_MOUSE |
| Immunogen Region: |
50-100 |
| Specificity: |
This blocking peptide is recommended for use in combination with SPHK1 antibody, STJ503118 |
| Immunogen: |
Synthetic peptide taken within amino acid region 50-100 on human SPHK1 protein. |
| Tissue Specificity | Widely expressed. Expressed in brain (at protein level). Detected in neurons. |
| Function | Catalyzes the phosphorylation of sphingosine to form sphingosine 1-phosphate (SPP), a lipid mediator with both intra- and extracellular functions. Also acts on D-erythro-sphingosine and to a lesser extent sphinganine, but not other lipids, such as D,L-threo-dihydrosphingosine, N,N-dimethylsphingosine, diacylglycerol, ceramide, or phosphatidylinositol. In contrast to proapoptotic SPHK2, has a negative effect on intracellular ceramide levels, enhances cell growth and inhibits apoptosis. Involved in the regulation of inflammatory response and neuroinflammation. Via the product sphingosine 1-phosphate, stimulates TRAF2 E3 ubiquitin ligase activity, and promotes activation of NF-kappa-B in response to TNF signaling. In response to TNF and in parallel to NF-kappa-B activation, negatively regulates RANTES induction through p38 MAPK signaling pathway. Involved in endocytic membrane trafficking induced by sphingosine, recruited to dilate endosomes, also plays a role on later stages of endosomal maturation and membrane fusion independently of its kinase activity. In Purkinje cells, seems to be also involved in the regulation of autophagosome-lysosome fusion upon VEGFA. Has serine acetyltransferase activity on PTGS2/COX2 in an acetyl-CoA dependent manner. The acetyltransferase activity increases in presence of the kinase substrate, sphingosine. During neuroinflammation, through PTGS2 acetylation, promotes neuronal secretion of specialized preresolving mediators (SPMs), especially 15-R-lipoxin A4, which results in an increase of phagocytic microglia. |
| Peptide Name | Sphingosine Kinase 1Sk 1Spk 1Acetyltransferase Sphk1 |
| Database Links | Reactome: R-MMU-1660661Reactome: -MMU-390471Reactome: -MMU-5218921Reactome: -MMU-9009391 |
| Cellular Localisation | CytoplasmEndosome MembranePeripheral Membrane ProteinNucleusCell MembraneSynapseTranslocated From The Cytoplasm To The Plasma Membrane In A Cib1-Dependent MannerBinds To Membranes Containing Negatively Charged Lipids But Not Neutral LipidsRecruited To Endocytic Membranes By Sphingosine Where Promotes Membrane Fusion |
| Alternative Peptide Names | Sphingosine Kinase 1 proteinSk 1 proteinSpk 1 proteinAcetyltransferase Sphk1 proteinSphk1 proteinSk1 protein |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
