Haemostasis | Research Area | St John's Laboratory

Western blot analysis of lysates from C6 cells, using Phospho-p53-S392 (STJ11102634) at 1:1000 dilution. C6 cells were treated by Paclitaxel (100 nM) at 37 °C for 20 hours. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 10s.

Anti-Phospho-TP53-S392 antibody [S4MR] (STJ11102634)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Rat

€183.13 - €803.13

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Western blot analysis of lysates from HeLa cells, using Phospho-p53-S46 Rabbit pAb (STJ29322) at 1:1000 dilution. HeLa cells were treated by Calyculin A (100 nM) at 37 °C for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 180s.

Anti-Phospho-TP53-S46 antibody (STJ29322)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IP/ELISA

Reactivity :

Human

€161.88 - €803.13

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Western blot analysis of lysates from MCF7 cells, using Phospho-p53-S6 Rabbit pAb (STJ11101083) at 1:1000 dilution. MCF7 cells were treated by ATP (5 mM) at 30 °C for 1 hour. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 90s.

Anti-Phospho-Tp53-S6 antibody (STJ11101083)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

€161.88 - €803.13

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Western blot analysis of lysates from HeLa cells, using Phospho-p53-S6 Rabbit pAb (STJ22435). <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA.

Anti-Phospho-TP53-S6 antibody (STJ22435)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

€161.88 - €803.13

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Western blot analysis of lysates from 293 and HT-29 cells, using Phospho-p53-S9 Rabbit pAb (STJ22434) at 1:1000 dilution. 293 cells were treated by UV for 15-30 minutes. HT-29 cells were treated by UV for 15-30 minutes. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 3min.

Anti-Phospho-TP53-S9 antibody (STJ22434)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IP/ELISA

Reactivity :

Human

€161.88 - €803.13

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Western blot analysis of lysates from NIH/3T3 cells, using Phospho-p53-S9 Rabbit mAb (STJ11102576) at 1:1000 dilution. NIH/3T3 cells were treated by UV at room temperature for 15-30 minutes. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Enhanced Kit <br/>Exposure time: 3min.

Anti-Phospho-TP53-S9 antibody [S6MR] (STJ11102576)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Mouse

€183.13 - €803.13

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Immunofluorescence analysis of rat-lung tissue. 1, p53 (phospho Ser15) Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Anti-Phospho-TP53-Ser15 antibody (1-50 aa) (STJ90365)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IP/IF/ELISA

Reactivity :

Human/Rat

€60.00 - €315.00

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Western blot analysis of various cells using Phospho-p53 (S20) Polyclonal Antibody

Anti-Phospho-TP53-Ser20 antibody (10-59 aa) (STJ90366)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat/Monkey

€60.00 - €315.00

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Western blot analysis of various cells using Phospho-p53 (S315) Polyclonal Antibody diluted at 1：1000

Anti-Phospho-TP53-Ser315 antibody (281-330 aa) (STJ90367)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/IP/ELISA

Reactivity :

Human/Rat/Mouse

€60.00 - €315.00

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Western blot analysis of various cells using Phospho-p53 (S33) Polyclonal Antibody diluted at 1：2000

Anti-Phospho-TP53-Ser33 antibody (1-50 aa) (STJ90368)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

€60.00 - €315.00

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Western blot analysis of HELA cells using Phospho-p53 (S366) Polyclonal Antibody

Anti-Phospho-TP53-Ser366 antibody (331-380 aa) (STJ90933)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

€60.00 - €315.00

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Western blot analysis of HELA Cell Lysate, antibody was diluted at 1:1000. Secondary antibody was diluted at 1:20000

Anti-Phospho-TP53-Ser366 antibody (STJ98965)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

€60.00 - €315.00

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Western blot analysis of various cells using Phospho-p53 (S37) Polyclonal Antibody diluted at 1：1000

Anti-Phospho-TP53-Ser37 antibody (11-60 aa) (STJ90441)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

€60.00 - €315.00

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Immunohistochemistry analysis of paraffin-embedded human brain, using p53 (Phospho-Ser376) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-TP53-Ser376 antibody (344-393 aa) (STJ91191)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

€60.00 - €315.00

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Western blot analysis of lysates from Jurkat cells treated with UV 15', using p53 (Phospho-Ser378) Antibody. The lane on the right is blocked with the phospho peptide.

Anti-Phospho-TP53-Ser378 antibody (344-393 aa) (STJ90574)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

€60.00 - €315.00

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Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using p53 (Phospho-Ser392) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-TP53-Ser392 antibody (344-393 aa) (STJ91044)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Monkey

€60.00 - €315.00

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Western blot of rat brain nuclear fraction lysate showing specific immunolabeling of the ~53 kDa p53 phosphorylated at Ser392 in the first lane (-). Phosphospecificity is shown in the second lane (+) where the immunolabeling is completely eliminated by blot treatment with lambda phosphatase ( Lambda-Ptase, 1200 units for 30 minutes).

Anti-Phospho-TP53-Ser392 antibody (STJA0003735)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Rat

€568.13

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Western blot analysis of various cells using Phospho-p53 (S46) Polyclonal Antibody diluted at 1：1000

Anti-Phospho-TP53-Ser46 antibody (11-60 aa) (STJ90369)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Monkey

€60.00 - €315.00

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Western blot analysis of various cells using Phospho-p53 (S6) Polyclonal Antibody diluted at 1：2000

Anti-Phospho-TP53-Ser6 antibody (1-50 aa) (STJ90370)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/IP/ELISA

Reactivity :

Human/Monkey

€60.00 - €315.00

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Western blot analysis of various cells using Phospho-p53 (S9) Polyclonal Antibody diluted at 1：2000

Anti-Phospho-TP53-Ser9 antibody (1-50 aa) (STJ90371)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Monkey

€60.00 - €315.00

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Anti-Phospho-TP53-T18 antibody (STJ11101084)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

€161.88 - €803.13

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Western blot analysis of lysates from MDA cells, using Phospho-p53-T18 Rabbit pAb (STJ22441). <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA.

Anti-Phospho-TP53-T18 antibody (STJ22441)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

€161.88 - €803.13

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Western blot analysis of lysates from 293T cells, using Phospho-p53-T55 Rabbit mAb (STJ11102577) at 1:1000 dilution. 293T cells were treated by CIP (20uL/400ul) at 37 °C for 1 hour or treated by Calyculin A (100 nM) at 37 °C for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 30s.

Anti-Phospho-TP53-T55 antibody [S7MR] (STJ11102577)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

€183.13 - €803.13

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Immunofluorescence analysis of rat-lung tissue. 1, p53 (phospho Thr18) Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Anti-Phospho-TP53-Thr18 antibody (1-50 aa) (STJ90372)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IF/WB/IHC/ELISA

Reactivity :

Human/Mouse/Rat

€60.00 - €315.00

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Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using p53 (Phospho-Thr387) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-TP53-Thr387 antibody (344-393 aa) (STJ91190)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

€60.00 - €315.00

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Immunohistochemistry analysis of paraffin-embedded human brain, using p53 (Phospho-Thr81) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-TP53-Thr81 antibody (51-100 aa) (STJ91132)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

€60.00 - €315.00

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Anti-Phospho-TUBA1C-Ser-165 antibody (STJA0005627)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Chicken/Human/Mouse/Rat/Xenopus

€459.38

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Anti-Phospho-TUBB3-Ser-172 antibody (STJA0005631)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ICC

Reactivity :

Chicken/Human/Mouse/Rat

€459.38

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Immunohistochemical analysis of paraffin-embedded human Squamous cell carcinoma of lung. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).

Anti-Phospho-VAV1-Tyr160 antibody (131-180 aa) (STJ91129)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

€60.00 - €315.00

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Western blot analysis of lysates from RAW264.7 cells, using VAV1 (Phospho-Tyr174) Antibody. The lane on the right is blocked with the phospho peptide.

Anti-Phospho-VAV1-Tyr174 antibody (141-190 aa) (STJ90434)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

€60.00 - €315.00

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Immunohistochemical analysis of paraffin-embedded Human breast cancer. Antibody was diluted at 1:100 (4°C overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.

Anti-Phospho-VAV1-Tyr174 antibody (STJ90433)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

€60.00 - €315.00

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Western blot analysis of lysates from Jurkat cells, using Phospho-VAV1-Y174 Rabbit pAb (STJ11105770). <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA.

Anti-Phospho-VAV1-Y174 antibody (STJ11105770)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Rat

€161.88 - €803.13

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Immunofluorescence analysis of NIH/3T3 cells, using VAV2 (Phospho-Tyr142) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-VAV2-Tyr142 antibody (108-157 aa) (STJ91109)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse

€60.00 - €315.00

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Western blot analysis of JK cells using Phospho-Vav3 (Y173) Polyclonal Antibody

Anti-Phospho-VAV3-Tyr173 antibody (STJ90999)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse

€60.00 - €315.00

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Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using Vinculin (Phospho-Tyr821) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-VCL-Tyr821 antibody (786-835 aa) (STJ91311)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

€60.00 - €315.00

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Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using WEE1 (Phospho-Ser53) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-WEE1-Ser53 antibody (19-68 aa) (STJ91224)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

€60.00 - €315.00

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Western blot analysis of 293 cells using Phospho-Wee1 (S642) Polyclonal Antibody cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).

Anti-Phospho-WEE1-Ser642 antibody (597-646 aa) (STJ90550)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

€60.00 - €315.00

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Western blot analysis of lysates from HeLa cells, using Phospho-YWHAZ-S58 Rabbit pAb (STJ22447). <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA.

Anti-Phospho-YWHAZ-S58 antibody (STJ22447)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC

Reactivity :

Human/Mouse/Rat

€161.88 - €803.13

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Western blot analysis of various cells using Phospho-14-3-3 Zeta (S58) Polyclonal Antibody diluted at 1：2000

Anti-Phospho-YWHAZ-Ser58 antibody (24-73 aa) (STJ91013)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

€60.00 - €315.00

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Western blot analysis of lysates from Jurkat cells treated with UV 15', using 14-3-3 zeta/delta (Phospho-Thr232) Antibody. The lane on the right is blocked with the phospho peptide.

Anti-Phospho-YWHAZ-Thr232 antibody (196-245 aa) (STJ90869)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

€60.00 - €315.00

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Western blot analysis of various lysates using PI3 Kinase p85 alpha Mouse mAb (STJ11106530) at 1:1000 dilution. <br/> Secondary antibody:HRP Goat Anti-Mouse IgG (H+L) (STJS000854) at 1:10000 dilution. <br/> Lysates/ proteins: 25 Mu g per lane. <br/> Blocking buffer: 3 % nonfat dry milk in TBST. <br/> Detection: ECL Basic Kit <br/> Exposure time: 30s.

Anti-PI3 Kinase p85 alpha antibody [SMM] (STJ11106530)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

€161.88 - €718.13

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Anti-PI3R6 antibody (590-640) (STJ506050)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human/Mouse

€534.38

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Anti-PI3R6 antibody (590-640) {Biotin} (STJ506051)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human/Mouse

€660.63

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Anti-PI3R6 antibody (590-640) {FITC} (STJ506052)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human/Mouse

€660.63

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Immunohistochemistry analysis of paraffin-embedded human gastric cancer using PICK1 antibody (STJ115535) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.

Anti-PICK1 antibody (300-400) (STJ115535)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC-P/ELISA

Reactivity :

Human

€140.63 - €655.63

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Western blot analysis of extracts of various cell lines, using PICK1 antibody (STJ24986) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Anti-PICK1 antibody (300-400) (STJ24986)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

€140.63 - €655.63

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Anti-PICK1 antibody (330-380) (STJ505932)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human/Mouse/Rat

€534.38

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Anti-PICK1 antibody (330-380) {Biotin} (STJ505933)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human/Mouse/Rat

€660.63

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