| Applications: |
CLIA |
| Reactivity: |
Rat |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
1.88pg/mL |
| Detection Limit: |
3.13~200pg/mL |
| Short Description: |
This rat PGF kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes. |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Chemiluminescence |
| Gene Symbol: |
Pgf |
| Gene ID: |
94203 |
| Uniprot ID: |
PLGF_RAT |
| Specificity: |
This kit recognizes Rat PGF in samples. No significant cross-reactivity or interference between Rat PGF and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Function | Growth factor active in angiogenesis and endothelial cell growth, stimulating their proliferation and migration. It binds to the receptor FLT1/VEGFR-1. Also promotes cell tumor growth. |
| Protein Name | Placenta Growth FactorPlgf |
| Database Links | Reactome: R-RNO-194313Reactome: -RNO-195399 |
| Cellular Localisation | Secreted |
| Alternative CLIA Names | Placenta Growth Factor CLIA kitPlgf CLIA kitPgf CLIA kitPlgf CLIA kit |
| Specificity | This kit recognizes Rat PGF in samples. No significant cross-reactivity or interference between Rat PGF and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro CLIA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent A | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Substrate Reagent B | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 99-111 | 104 |
| EDTA plasma(n=8) | 85-98 | 91 |
| Cell culture media(n=8) | 97-111 | 103 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 9.38 | 23.95 | 97.83 | 8.91 | 22.41 | 100.36 |
| Standard deviation | 0.97 | 2.16 | 7.87 | 0.87 | 2.20 | 8.68 |
| CV (%) | 10.34 | 9.02 | 8.04 | 9.76 | 9.82 | 8.65 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
