| Function | Growth factor active in angiogenesis and endothelial cell growth, stimulating their proliferation and migration. It binds to the receptor FLT1/VEGFR-1. Also promotes cell tumor growth. |
| Protein Name | Placenta Growth FactorPlgf |
| Database Links | Reactome: R-RNO-194313Reactome: -RNO-195399 |
| Cellular Localisation | Secreted |
| Alternative CLIA Names | Placenta Growth Factor CLIA kitPlgf CLIA kitPgf CLIA kitPlgf CLIA kit |
| Specificity | This kit recognizes Rat PGF in samples. No significant cross-reactivity or interference between Rat PGF and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro CLIA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent A | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Substrate Reagent B | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 99-111 | 104 |
| EDTA plasma(n=8) | 85-98 | 91 |
| Cell culture media(n=8) | 97-111 | 103 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 9.38 | 23.95 | 97.83 | 8.91 | 22.41 | 100.36 |
| Standard deviation | 0.97 | 2.16 | 7.87 | 0.87 | 2.20 | 8.68 |
| CV (%) | 10.34 | 9.02 | 8.04 | 9.76 | 9.82 | 8.65 |
