| Applications: | CLIA |
| Reactivity: | Rat |
| Note: | FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: | 7.5ng/mL |
| Detection Limit: | 12.5~800ng/mL |
| Short Description : | This rat ApoB kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes in serum, plasma and other biological fluids. |
| Storage Instruction: | If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: | 3.5h |
| Detection: | Chemiluminescence |
| Gene Symbol: | Apob |
| Gene ID: | 54225 |
| Uniprot ID: | APOB_RAT |
| Specificity: | This kit recognizes Rat ApoB in samples. No significant cross-reactivity or interference between Rat ApoB and analogues was observed. |
| Sample Type: | Serum, plasma and other biological fluids |
| Tissue Specificity | Detected in intestine and liver (at protein level). |
| Post Translational Modifications | Palmitoylated.structural requirement for proper assembly of the hydrophobic core of the lipoprotein particle. |
| Function | Apolipoprotein B is a major protein constituent of chylomicrons (apo B-48), LDL (apo B-100) and VLDL (apo B-100). Apo B-100 functions as a recognition signal for the cellular binding and internalization of LDL particles by the apoB/E receptor. |
| Protein Name | Apolipoprotein B-100 Apo B-100 Cleaved Into - Apolipoprotein B-48 Apo B-48 |
| Database Links | Reactome: R-RNO-202733 Reactome: -RNO-3000471 Reactome: -RNO-3000480 Reactome: -RNO-3000497 Reactome: -RNO-381426 Reactome: -RNO-432142 Reactome: -RNO-5686938 Reactome: -RNO-8856825 Reactome: -RNO-8856828 Reactome: -RNO-8866423 Reactome: -RNO-8957275 Reactome: -RNO-8963888 Reactome: -RNO-8963901 Reactome: -RNO-8964026 Reactome: -RNO-8964038 Reactome: -RNO-8964046 Reactome: -RNO-9707616 Reactome: -RNO-975634 |
| Cellular Localisation | Cytoplasm Secreted Lipid Droplet |
| Alternative CLIA Names | Apolipoprotein B-100 CLIA kit Apo B-100 Cleaved Into - Apolipoprotein B-48 CLIA kit Apo B-48 CLIA kit Apob CLIA kit Aa1064 CLIA kit Ac1-060 CLIA kit |
| Specificity | This kit recognizes Rat ApoB in samples. No significant cross-reactivity or interference between Rat ApoB and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
|---|---|---|
| Micro CLIA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent A | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Substrate Reagent B | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy |
| Sample Type | Range (%) | Average Recovery (%) |
|---|---|---|
| Serum(n=8) | 97-111 | 104 |
| EDTA plasma(n=8) | 84-99 | 91 |
| Cell culture media(n=8) | 90-103 | 96 |
| Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision | |
|---|---|---|---|---|---|---|
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (ng/mL) | 38.99 | 72.23 | 335.06 | 42.28 | 70.51 | 322.12 |
| Standard deviation | 3.32 | 5.42 | 26.37 | 3.80 | 5.38 | 33.47 |
| CV (%) | 8.52 | 7.50 | 7.87 | 8.99 | 7.63 | 10.39 |