| Applications: | WB |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | PTP alpha Positive Control is synthetically produced from the sequence and is suitable for use in western blot applications. |
| Formulation: | Provided as 100 uL ready-to-use, in SDS-PAGE sample buffer (Laemelli's buffer) containing Tris, pH 6.8, 1 % SDS, Glycerol and Bromophenolblue blue as tracking dye. The sample is reduced by adding 2% beta mercaptoethanol. The protein concentration is |
| Dilution Range: | WB: 1:2, 500-1:5, 000 |
| Storage Instruction: | Store at-20°C for long term storage. Avoid freeze-thaw cycles. |
| Gene Symbol: | PTPRA |
| Gene ID: | 5786 |
| Uniprot ID: | PTPRA_HUMAN |
| Immunogen: | Receptor-type tyrosine-protein phosphatase Alpha |
| Post Translational Modifications | Integrin binding to extracellular matrix induces phosphorylation at Tyr-798 which induces PTPRA localization and recruitment of BCAR3, BCAR1 and CRK to focal adhesions. |
| Function | Tyrosine protein phosphatase which is involved in integrin-mediated focal adhesion formation. Following integrin engagement, specifically recruits BCAR3, BCAR1 and CRK to focal adhesions thereby promoting SRC-mediated phosphorylation of BRAC1 and the subsequent activation of PAK and small GTPase RAC1 and CDC42. |
| Peptide Name | Receptor-Type Tyrosine-Protein Phosphatase Alpha Protein-Tyrosine Phosphatase Alpha R-Ptp-Alpha |
| Database Links | Reactome: R-HSA-375165 Reactome: R-HSA-5673001 |
| Cellular Localisation | Cell Membrane Single-Pass Type I Membrane Protein Cell Junction Focal Adhesion Localizes To Focal Adhesion Sites Following Integrin Engagement |
| Alternative Peptide Names | Receptor-Type Tyrosine-Protein Phosphatase Alpha protein Protein-Tyrosine Phosphatase Alpha protein R-Ptp-Alpha protein PTPRA protein PTPA protein PTPRL2 protein |
Information sourced from Uniprot.org